Anti-Mouse Ly-6G Antibody, Clone 1A8

Rat monoclonal IgG2a antibody against mouse Ly-6G

Anti-Mouse Ly-6G Antibody, Clone 1A8

Rat monoclonal IgG2a antibody against mouse Ly-6G

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Rat monoclonal IgG2a antibody against mouse Ly-6G
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Overview

The 1A8 antibody reacts with Ly-6G, a 21 - 25 kDa GPI-anchored protein, which together with the structurally related Ly-6C protein comprises the granulocyte receptor-1 antigen (Gr-1). Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. Monocytes in the bone marrow transiently express Gr-1 during development and the expression level is strongly correlated with granulocyte differentiation and maturation. In the periphery, Gr-1 is found predominantly on neutrophils and is a useful marker for these cells. The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762).
Subtype
Primary Antibodies
Target Antigen
Ly-6G
Alternative Names
Gr-1, GR1, Ly6G
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, Pacific Blue, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Mouse
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
1A8
Gene ID
546644
Isotype
IgG2a, kappa

Data Figures

Data for FITC-Conjugated

Figure 1. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, FITC (filled histogram) or a rat IgG2a, kappa FITC isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram). (B) Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse uterine tissue labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, followed by anti-mouse, HRP. DAB substrate was used for visualization.

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 6. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 7. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse Ly-6G Antibody, Clone 1A8, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60031BT, 60031BT.1
Lot #
All
Language
English
Catalog #
60031FI, 60031FI.1
Lot #
All
Language
English
Catalog #
60031AZ.1, 60031AZ
Lot #
All
Language
English
Catalog #
60031
Lot #
All
Language
English
Catalog #
60031PS.1, 60031PS
Lot #
All
Language
English
Catalog #
60031PB.1, 60031PB
Lot #
All
Language
English
Catalog #
60031PE, 60031PE.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031BT, 60031BT.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031FI, 60031FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031AZ.1, 60031AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031PS.1, 60031PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031PB.1, 60031PB
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60031PE, 60031PE.1
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.