Anti-Mouse CD11b Antibody, Clone M1/70

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
From: 69 USD

Options

* Required Fields

Catalog # (Select a product)
Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
From: 69 USD

Overview

The M1/70 antibody reacts with CD11b, an ~170 kDa type 1 transmembrane glycoprotein which associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen, Mac-1 functions in several processes, including the adherence of neutrophils and monocytes to stimulated endothelium and phagocytosis of complement-coated particles. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. CD11b is a relatively late marker for myeloid differentiation and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The M1/70 antibody reportedly blocks iC3b binding to Mac-1.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Isolation Kit (Catalog #19861).
Subtype:
Primary Antibodies
Target Antigen:
CD11b
Alternative Names:
alphaM integrin, C3biR, CR3, Ly-40, Mac-1, Mo1
Reactive Species:
Baboon; Chimpanzee; Cynomolgus; Human; Mouse; Rabbit; Rhesus
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated; Pacific Blue
Host Species:
Rat
Cell Type:
Granulocytes and Subsets; Monocytes
Application:
Cell Isolation; CyTOF; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation
Area of Interest:
Immunology
Clone:
M1/70
Gene ID:
16409
Isotype:
IgG2b, kappa

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Educational Materials

(5)

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa biotin isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.