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Anti-Mouse CD11b Antibody, Clone M1/70

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b

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Anti-Mouse CD11b Antibody, Clone M1/70

Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b

From: 50 USD
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Rat monoclonal IgG2b antibody against human, mouse, rhesus CD11b
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Overview

The M1/70 antibody reacts with CD11b, an ~170 kDa type 1 transmembrane glycoprotein which associates non-covalently with CD18 to form the heterodimeric Mac-1 receptor. Through its interactions with ligands such as ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen, Mac-1 functions in several processes, including the adherence of neutrophils and monocytes to stimulated endothelium and phagocytosis of complement-coated particles. CD11b is expressed on the surface of granulocytes, monocytes, NK cells, dendritic cells, tissue macrophages, and subsets of T and B cells, and has been used as a marker to distinguish naïve and memory CD8+ T cells. CD11b is a relatively late marker for myeloid differentiation and is undetectable on most myelomonocytic hematopoietic progenitor cells and more primitive cells. The M1/70 antibody reportedly blocks iC3b binding to Mac-1. This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Monocyte Isolation Kit (Catalog #19861).
Subtype
Primary Antibodies
Target Antigen
CD11b
Alternative Names
alphaM integrin, C3biR, CR3, Ly-40, Mac-1, Mo1
Reactive Species
Baboon, Chimpanzee, Human, Rabbit, Rhesus
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Granulocytes and Subsets, Monocytes
Species
Human, Mouse, Non-Human Primate, Other
Application
Cell Isolation, CyTOF, FACS, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blotting
Area of Interest
Immunology
Clone
M1/70
Gene ID
16409
Isotype
IgG2b, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488 and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE and anti-mouse CD45 APC.
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa PE isotype control antibody and anti-mouse CD45 APC.
(C) Flow cytometry analysis of C57BL/6 mouse splenocytes processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of splenocytes (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).
(D) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse Monocyte Enrichment Kit and labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PE. Histograms show labeling of bone marrow cells (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2b, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, followed by a mouse anti-rat IgG2b antibody, FITC (filled histogram), or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa APC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Biotin followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa biotin isotype control antibody followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).
(B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa FITC isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a rat IgG2b, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS.1, 60001PS
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 60001BT, 100-0438
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0434, 100-0435
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ.1, 60001AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
60001FI, 100-0436, 100-0437
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PerCP-Cy5.5
Catalog #
60001PS.1, 60001PS
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Alexa Fluor® 488
Catalog #
60001AD, 60001AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Biotin
Catalog #
100-0439, 60001BT, 100-0438
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, PE
Catalog #
100-0434, 100-0435
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, APC
Catalog #
60001AZ.1, 60001AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, Pacific Blue™
Catalog #
60001PB, 60001PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70
Catalog #
100-0433
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD11b Antibody, Clone M1/70, FITC
Catalog #
60001FI, 100-0436, 100-0437
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (4)

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Hematopoietic Stem and Progenitor Cells - Products for Your Research
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Legal Statement:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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