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Anti-Mouse Sca1 Antibody, Clone E13-161.7

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E)

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Anti-Mouse Sca1 Antibody, Clone E13-161.7

Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E)

From: 106 USD
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Rat monoclonal IgG2a antibody against mouse Sca1 (Ly-6A/E)
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Overview

The E13-161.7 antibody reacts with Sca1 (Stem Cell Antigen-1 or Ly-6A/E), an 18 kDa GPI-linked protein belonging to the lymphocyte activation protein-6 (Ly-6) family. Sca1 is expressed on the surface of hematopoietic stem and progenitor cells, myeloid cells, and peripheral B and T lymphocytes. Sca1 is expressed by mice with either the Ly-6.1 or Ly-6.2 allotypes but the pattern of expression differs in the circulating cell population according to the allotype. Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) possess relatively high numbers of Sca1+ resting lymphocytes compared to Ly-6.1 strains (e.g. A, BALB/c, CBA, C3H/He, DBA/1, NZB). Sca1 expression levels are strongly upregulated in all strains upon cellular activation. Sca1 is involved in the regulation of T and B cell responses and is believed to play roles in the differentiation, proliferation, and survival of a variety of stem cells. Sca1 has emerged as a phenotypic marker of choice for identifying and isolating hematopoietic stem and progenitor cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse SCA1 Biotin Positive Selection Kit (Catalog #18856).
Subtype
Primary Antibodies
Target Antigen
Sca1 (Ly-6A/E)
Alternative Names
Ly-6A/E, Sca-1
Reactive Species
Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
Hematopoietic Stem and Progenitor Cells, Mammary Cells
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Clone
E13-161.7
Gene ID
110454
Isotype
IgG2a, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 (filled histogram) or a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells pre-labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488 and processed with the EasySep™ Mouse SCA1 Positive Selection Kit. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa Alexa Fluor® 488 isotype control antibody is shown in the bottom panel (open histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE (filled histogram) or a rat IgG2a, kappa PE isotype control antibody (open histogram).
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ PE Selection Kit for Mouse Cells (Catalog #18554) using Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE. Histograms show labeling of bone marrow (Start) and isolated cells (Isolated). Labeling of start cells with a rat IgG2a, kappa PE isotype control antibody is shown in the bottom panel (open histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC (filled histogram) or a rat IgG2a, kappa isotype control antibody, APC (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin followed by streptavidin (SAV) APC (filled histogram) or a biotinylated rat IgG2a, kappa isotype control antibody followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC (filled histogram) or a rat IgG2a, kappa isotype control antibody, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7
Catalog #
60032
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE
Catalog #
60032PE.1, 60032PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC
Catalog #
60032AZ.1, 60032AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin
Catalog #
60032BT
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488
Catalog #
60032AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC
Catalog #
60032FI.1, 60032FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7
Catalog #
60032
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, PE
Catalog #
60032PE.1, 60032PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, APC
Catalog #
60032AZ.1, 60032AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Biotin
Catalog #
60032BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, Alexa Fluor® 488
Catalog #
60032AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse Sca1 Antibody, Clone E13-161.7, FITC
Catalog #
60032FI.1, 60032FI
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (3)

Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Reliable Antibodies for Your Research
Brochure
Reliable Antibodies for Your Research

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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