TeSR™-E8™

Feeder-free, xeno-free culture medium for maintenance of human ES and iPS cells

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TeSR™-E8™ Kit for hESC/hiPSC Maintenance

Feeder-free, xeno-free culture medium for maintenance of human ES and iPS cells

500 mL Kit
Catalog #05940
209 USD

Overview

TeSR™-E8™ is a feeder-free, xeno-free culture medium for human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. It is based on the E8 formulation developed by the laboratory of Dr. James Thomson (University of Wisconsin-Madison), the lead research group behind the design of mTeSR™1, the most widely-published feeder-free culture medium for pluripotent stem cells.

Like mTeSR™1, TeSR™-E8™ medium is made with the highest level of quality and care. It contains only the essential components required for maintenance of ES and iPS cells, providing a simpler medium for the culture of pluripotent stem cells. TeSR™-E8™ can be used with Corning® Matrigel® hESC-qualified matrix, or, for a completely xeno-free system, Vitronectin XF™ .
Advantages:
Simplified, low-protein formulation based on the popular mTeSR™ 1 medium for maintaining human ES and iPS cells
Components:
    • TeSR™-E8™ Basal Medium, 474 mL
    • TeSR™-E8™ 20X Supplement, 25 mL
    • TeSR™-E8™ 500X Supplement, 1 mL
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Expansion; Maintenance
Brand:
TeSR
Area of Interest:
Stem Cell Biology
Formulation:
Serum-Free; Defined

Scientific Resources

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

High Expansion Rates are Observed in TeSR™-E8™ Cultures

Figure 1. High Expansion Rates are Observed in TeSR™-E8™ Cultures

Graph shows the average fold expansion per passage +/- SEM obtained for human ES and iPS cells cultured in TeSR™-E8™ with Corning® Matrigel® over 10 passages (orange). Representative data is also shown for human ES cells cultured in mTeSR™1 using a similar protocol for comparison (Gray). Expansion was determined by enumerating the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded. Note that this data is representative of cultures passaged after 6-7 days in culture, lower expansion should be expected if using shorter culture times.

Normal Human ES and iPS Cell Morphology is Observed in TeSR™-E8™ Cultures

Figure 2. Normal Human ES and iPS Cell Morphology is Observed in TeSR™-E8™ Cultures

Undifferentiated human ES (H1) and iPS (A13700) cells cultured on Corning® Matrigel® Matrix in TeSR™-E8™ retain the prominent nucleoli and high nuclear-to-cytoplasm ratio characteristic of this cell type. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.

Cells Cultured in TeSR™-E8™ Medium Express Undifferentiated Cell Markers

Figure 3. Cells Cultured in TeSR™-E8™ Medium Express Undifferentiated Cell Markers

(A) Histogram analysis for H1 and H9 human ES cells characterized using FACS for undifferentiated cell markers (SSEA3 and OCT4) after 10 passages in TeSR™-E8™ (blue = sample, black = secondary antibody only). (B) H1 cells cultured in TeSR™-E8™ medium were characterized by OCT4 immunostaining after 18 passages.

Uniformly-sized Embryoid Bodies Differentiated from Cells Cultured in TeSR™-E8™

Figure 4. Uniformly-Sized Embryoid Bodies Differentiated from Cells Cultured in TeSR™-E8™

(A) H1 cells cultured using TeSR™-E8™ on Corning® Matrigel® were dissociated to single cells using standard techniques, then placed in an AggreWell™ 400 plate containing AggreWell™ medium and 10 µM Y-27632 for 24 hours, (B) after which they were transferred to an ultra low-adherence (ULA) plate for inspection.

Publications

(29)
Stem cell research 2017 MAY

Establishment of an induced pluripotent stem cell (iPSC) line from a 9-year old male with autism spectrum disorder (ASD).

Varga E et al.

Abstract

Peripheral blood mononuclear cells (PBMCs) were collected from a clinically characterized patient with autism spectrum disorder (ASD). The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus delivery system. The pluripotency of transgene-free iPSCs was verified by immunocytochemistry for pluripotency markers and by spontaneous in vitro differentiation towards the 3 germ layers. Furthermore, the iPSC line showed normal karyotype. Our model might offer a good platform to study the pathomechanism of ASD, also for drug testing, early biomarker discovery and gene therapy studies.
Cell stem cell 2017 MAR

Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States.

Collier AJ et al.

Abstract

Human pluripotent stem cells (PSCs) exist in naive and primed states and provide important models to investigate the earliest stages of human development. Naive cells can be obtained through primed-to-naive resetting, but there are no reliable methods to prospectively isolate unmodified naive cells during this process. Here we report comprehensive profiling of cell surface proteins by flow cytometry in naive and primed human PSCs. Several naive-specific, but not primed-specific, proteins were also expressed by pluripotent cells in the human preimplantation embryo. The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations. This analysis revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation. Thus, identification of state-specific proteins provides a robust set of molecular markers to define the human PSC state and allows new insights into the molecular events leading to naive cell resetting.
Stem cell research 2017 APR

Rapid establishment of the European Bank for induced Pluripotent Stem Cells (EBiSC) - the Hot Start experience.

De Sousa PA et al.

Abstract

A fast track Hot Start" process was implemented to launch the European Bank for Induced Pluripotent Stem Cells (EBiSC) to provide early release of a range of established control and disease linked human induced pluripotent stem cell (hiPSC) lines. Established practice amongst consortium members was surveyed to arrive at harmonised and publically accessible Standard Operations Procedures (SOPs) for tissue procurement�
Developmental cell 2017 APR

Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors.

Krentz NAJ et al.

Abstract

During pancreatic development, proliferating pancreatic progenitors activate the proendocrine transcription factor neurogenin 3 (NEUROG3), exit the cell cycle, and differentiate into islet cells. The mechanisms that direct robust NEUROG3 expression within a subset of progenitor cells control the size of the endocrine population. Here we demonstrate that NEUROG3 is phosphorylated within the nucleus on serine 183, which catalyzes its hyperphosphorylation and proteosomal degradation. During progression through the progenitor cell cycle, NEUROG3 phosphorylation is driven by the actions of cyclin-dependent kinases 2 and 4/6 at G1/S cell-cycle checkpoint. Using models of mouse and human pancreas development, we show that lengthening of the G1 phase of the pancreatic progenitor cell cycle is essential for proper induction of NEUROG3 and initiation of endocrine cell differentiation. In sum, these studies demonstrate that progenitor cell-cycle G1 lengthening, through its actions on stabilization of NEUROG3, is an essential variable in normal endocrine cell genesis.
Cell metabolism 2016 SEP

$$-Ketoglutarate Accelerates the Initial Differentiation of Primed Human Pluripotent Stem Cells.

TeSlaa T et al.

Abstract

Pluripotent stem cells (PSCs) can self-renew or differentiate from naive or more differentiated, primed, pluripotent states established by specific culture conditions. Increased intracellular $$-ketoglutarate ($$KG) was shown to favor self-renewal in naive mouse embryonic stem cells (mESCs). The effect of $$KG or $$KG/succinate levels on differentiation from primed human PSCs (hPSCs) or mouse epiblast stem cells (EpiSCs) remains unknown. We examined primed hPSCs and EpiSCs and show that increased $$KG or $$KG-to-succinate ratios accelerate, and elevated succinate levels delay, primed PSC differentiation. $$KG has been shown to inhibit the mitochondrial ATP synthase and to regulate epigenome-modifying dioxygenase enzymes. Mitochondrial uncoupling did not impede $$KG-accelerated primed PSC differentiation. Instead, $$KG induced, and succinate impaired, global histone and DNA demethylation in primed PSCs. The data support $$KG promotion of self-renewal or differentiation depending on the pluripotent state.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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