TeSR™-E8™

Feeder-free, animal component-free culture medium for maintenance of human ES and iPS cells

TeSR™-E8™

Feeder-free, animal component-free culture medium for maintenance of human ES and iPS cells

TeSR™-E8™ Kit for hESC/hiPSC Maintenance
1 Kit
273 USD
Catalog # 05990

Feeder-free, animal component-free culture medium for maintenance of human ES and iPS cells

Product Advantages


  • Simplified, low-protein formulation based on the popular mTeSR™1 medium for maintaining human ES and iPS cells

What's Included

  • TeSR™-E8™ Basal Medium, 480 mL
  • TeSR™-E8™ 25X Supplement, 20 mL

Overview

TeSR™-E8™ is a feeder-free, animal component-free culture medium for human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. It is based on the E8 formulation developed by the laboratory of Dr. James Thomson (University of Wisconsin-Madison), the lead research group behind the design of mTeSR™1, the most widely published feeder-free culture medium for pluripotent stem cells.

Like mTeSR™1, TeSR™-E8™ medium is made with the highest level of quality and care. It contains only the essential components required for maintenance of ES and iPS cells, providing a simpler medium for the culture of pluripotent stem cells. TeSR™-E8™ may be used with either Corning® Matrigel® hESC-Qualified Matrix (Catalog #07181) or, for a completely defined xeno-free system, Vitronectin XF™ (Catalog #07180, a matrix developed and manufactured by Nucleus Biologics) as the culture matrix.
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion, Maintenance
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation
Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05990
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
TeSR™-E8™
Catalog #
05990
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05990
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05990
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (17)

Publications (4)

A Novel Toolkit for Characterizing the Mechanical and Electrical Properties of Engineered Neural Tissues. M. Robinson et al. Biosensors 2019 apr

Abstract

We have designed and validated a set of robust and non-toxic protocols for directly evaluating the properties of engineered neural tissue. These protocols characterize the mechanical properties of engineered neural tissues and measure their electrophysical activity. The protocols obtain elastic moduli of very soft fibrin hydrogel scaffolds and voltage readings from motor neuron cultures. Neurons require soft substrates to differentiate and mature, however measuring the elastic moduli of soft substrates remains difficult to accurately measure using standard protocols such as atomic force microscopy or shear rheology. Here we validate a direct method for acquiring elastic modulus of fibrin using a modified Hertz model for thin films. In this method, spherical indenters are positioned on top of the fibrin samples, generating an indentation depth that is then correlated with elastic modulus. Neurons function by transmitting electrical signals to one another and being able to assess the development of electrical signaling serves is an important verification step when engineering neural tissues. We then validated a protocol wherein the electrical activity of motor neural cultures is measured directly by a voltage sensitive dye and a microplate reader without causing damage to the cells. These protocols provide a non-destructive method for characterizing the mechanical and electrical properties of living spinal cord tissues using novel biosensing methods.
Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States. Collier AJ et al. Cell stem cell 2017 MAR

Abstract

Human pluripotent stem cells (PSCs) exist in naive and primed states and provide important models to investigate the earliest stages of human development. Naive cells can be obtained through primed-to-naive resetting, but there are no reliable methods to prospectively isolate unmodified naive cells during this process. Here we report comprehensive profiling of cell surface proteins by flow cytometry in naive and primed human PSCs. Several naive-specific, but not primed-specific, proteins were also expressed by pluripotent cells in the human preimplantation embryo. The upregulation of naive-specific cell surface proteins during primed-to-naive resetting enabled the isolation and characterization of live naive cells and intermediate cell populations. This analysis revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation. Thus, identification of state-specific proteins provides a robust set of molecular markers to define the human PSC state and allows new insights into the molecular events leading to naive cell resetting.
Peripheral blood derived induced pluripotent stem cells (iPSCs) from a female with familial hypertrophic cardiomyopathy. S. B. Ross et al. Stem cell research 2017

Abstract

Induced pluripotent stem cells (iPSCs) were generated from peripheral blood mononuclear cells (PBMCs) obtained from a 62-year-old female with familial hypertrophic cardiomyopathy (HCM). PBMCs were reprogrammed to a pluripotent state following transfection with non-integrative episomal vectors carrying reprogramming factors OCT4, SOX2, LIN28, KLF4 and L-MYC. iPSCs were shown to express pluripotency markers, possess trilineage differentiation potential, carry rare variants identified in DNA isolated directly from the patient's whole blood, have a normal karyotype and no longer carry episomal vectors for reprogramming. This line is a useful resource for identifying unknown genetic causes of HCM.

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