The liver is a vital organ involved in a wide range of processes such as detoxification, protein synthesis, metabolism, and hormone production. Despite an astounding regenerative capacity in vivo, expansion of liver cells in vitro has remained a challenge. Growth of the hepatic epithelium as organoids provides researchers with a stable population of liver cells for research in a variety of fields including liver biology, disease modeling and toxicity screening.
Explore the resources below for information on the expansion and maintenance of hepatic epithelial cells as organoids.
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Key Applications of Hepatic Organoids
Adult Tissue-Derived Liver Organoids
Huch M, et al. (2015) Long-term culture of genome-stable bipotent stem cells from adult human liver. Cell. 160(1-2): 299-312.
Huch M, et al. (2013) In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration. Nature. 494(7436): 247-50.
Kuijk EW, et al. (2016) Generation and characterization of rat liver stem cell lines and their engraftment in a rat model of liver failure. Sci Rep. 6: 22154.
Nantasanti S, et al. (2015) Disease modeling and gene therapy of copper storage disease in canine hepatic organoids. Stem Cell Reports. 5(5): 895-907.
Disease Modeling and Cancer Research
Broutier L, et al. (2017) Human primary liver cancer-derived organoid cultures for disease modelling and drug screening. Nat Med. 23(12): 1424-1435.
Sampaziotis F, et al. (2015) Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation. Nat Biotechnol. 33(8): 845-852.
Hindley CJ, et al. (2016) Organoids from adult liver and pancreas: Stem cell biology and biomedical utility. Dev Biol. 420(2): 251-261.
Hepatic Cell Biology
Tarlow BD, et al. (2014) Clonal tracing of Sox9+ liver progenitors in mouse oval cell injury. Hepatology. 60(1): 278-89.
Yimlamai D, et al. (2014) Hippo pathway activity influences liver cell fate. Cell. 157(6): 1324-38.
Kruitwagen HS, et al. (2018) DYRK1A Is a Regulator of S-Phase Entry in Hepatic Progenitor Cells. Stem Cells Dev.
hPSC-Derived Liver Organoids
Takebe T, et al. (2017) Massive and reproducible production of liver buds entirely from human pluripotent stem cells. Cell Rep. 21(10):2661-2670.
Takebe T, et al. (2014) Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. Nat Protoc. 9(2): 396-409.
Organoids in Multi-Lineage Model Systems
Takebe T, et al. (2012) Self-Organization of human hepatic organoid by recapitulating organogenesis in vitro. Transplant Proc. 44(4): 1018-20.
Skardal A, et al. (2015) Liver-tumor hybrid organoids for modeling tumor growth and drug response in vitro. Ann Biomed Eng. 43(10): 2361-73.