HepatiCult™ Organoid Growth Medium (Mouse)

Cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids
HepatiCult™ Organoid Growth Medium (Mouse)

Cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids

1 Kit
Catalog # 06030
299 USD
Required Products
  1. AggreWell™ Rinsing Solution
    Anti-Adherence Rinsing Solution

    Rinsing solution for cultureware to prevent cell adhesion

  2. Dispase (1 U/mL)
    Dispase (1 U/mL)

    1 U/mL dispase in DMEM/F-12

  3. Collagenase Type IV (1 mg/mL)
    Collagenase Type IV (1 mg/mL)

    Cell dissociation reagent

Overview

HepatiCult™ Organoid Growth Medium (Mouse) is a serum-free, defined cell culture medium for establishment and maintenance of mouse hepatic progenitor organoids. These organoids, or "mini-livers", provide an in vitro organotypic culture system for studying hepatic stem and progenitor cells. Organoids grown in HepatiCult™ feature an epithelium expressing genes marking hepatic stem and progenitor cells (PROM1, AXIN2, SOX9 and CD44), ducts (KRT19 and HNF1b) and hepatocytes (HNF4a, AFP). Hepatic organoids can be passaged every 4 - 7 days, can be cryopreserved, and are primed for downstream differentiation.

HepatiCult™ supports mouse hepatic organoid culture either embedded in Corning® Matrigel® domes or in a dilute Matrigel® suspension. Organoid culture enables convenient in vitro characterization of the hepatic epithelium in a physiologically relevant system and reduces the need for animal use.
Advantages
• Convenient in vitro system for generating organoids in 4 - 5 days
• Step-by-step protocol with no injury models, hand-picking of ducts, or cell sorting required
• Simple, two-component format; serum-free and defined medium formulation
• Flexible protocol for generation and maintenance of organoid cultures in matrix domes or suspension culture
Components
• HepatiCult™ OGM Mouse Basal Medium, 95 mL
• HepatiCult™ OGM Mouse Supplement, 5 mL
Subtype
Specialized Media
Cell Type
Hepatic Cells
Species
Mouse
Application
Cell Culture, Expansion, Maintenance, Organoid Culture
Brand
HepatiCult
Area of Interest
Cancer Research, Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
HepatiCult™ Organoid Growth Medium (Mouse)
Catalog #
06030
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
HepatiCult™ Organoid Growth Medium (Mouse)
Catalog #
06030
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
HepatiCult™ Organoid Growth Medium (Mouse)
Catalog #
06030
Lot #
All
Language
English

Educational Materials(17)

Brochure
Organoids
Brochure
2019-2020 Cell Culture Training Catalog
Brochure
HepatiCult™ Organoid Growth Medium (Mouse)
Technical Bulletin
Mouse Hepatic Progenitor Organoid Culture: Supplementary Protocols
Wallchart
SnapShot: Growing Organoids from Stem Cells
Video
1:07
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
Video
1:18
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
Webinar
1:10:09
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Webinar
52:35
Organoid Expert Panel
Webinar
1:03:40
From Stem Cells to Organoids: Modeling the Gastrointestinal Tract
Webinar
41:47
Challenges in Translating iPSC Technology
Webinar
50:59
Adding Hepatic Organoids to Your Research
Webinar
24:56
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Webinar
1:14:36
Re-Creating Disease with Kidney Organoids and CRISPR
Scientific Poster
Culture and Differentiation of Mouse Hepatic Organoids Using Serum-free HepatiCult™ Organoid Growth Medium
Interview
Luigi Aloia, PhD
Interview
Meritxell Huch, PhD
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids

Data and Publications

Data

Figure 1. Mouse Hepatic Progenitor Organoids can be Initiated from a Variety of Starting Materials

HepatiCult™ Organoid Growth Medium (Mouse) enables the initiation of hepatic progenitor organoids from (A) duct fragments, (B) single cells or (C) cryopreserved organoids. All organoids were grown in Matrigel® domes and imaged on day 7 of primary culture or the first passage post thaw (cryopreserved organoids).

Figure 2. Hepatic Organoids can be Grown in Matrigel® Domes or as a Dilute Matrigel® Suspension

Hepatic progenitor organoids were cultured from freshly isolated mouse hepatic duct fragments in HepatiCult™ Organoid Growth Medium (Mouse) and plated (A) in Matrigel® domes or (B) as a dilute Matrigel® suspension. Organoids grown in either culture condition are ready for passage within 4-7 days.

Figure 3. Organoids grown in HepatiCult™ Organoid Growth Medium (Mouse) Display Some Characteristics Typical of the Mature Hepatic Epithelium

(A) Hepatic progenitor organoids exhibit the polygonal morphology typical of the hepatic epithelium. (B) Hepatic progenitors cultured as organoids show binucleation (arrows), a feature common of mature hepatocytes. (C) Immunocytochemistry analysis shows localization of MRP4 (green), a membrane-bound, unidirectional efflux transporter, along the exterior of the organoids and DAPI (red) localized to the cellular nuclei. This indicates cellular polarization of the organoids with the basolateral surface of the epithelium distal from the lumen. (D) Hepatic organoids contain an actively dividing progenitor population, seen through the expression of Ki67 (red). Cell nuclei are stained with DAPI (blue).

Figure 4. Analysis of Organoid Gene Expression when Grown in Matrigel® Domes and as a Dilute Matrigel® Suspension

Analysis of marker expression by RNA-seq shows organoids grown in HepatiCult™ Organoid Growth Medium (Mouse) in either Matrigel® domes or in a dilute Matrigel® suspension express markers associated with hepatic stem and progenitor cells. The organoids also express low levels of genes associated with mature hepatic cell types, including cholangiocytes and hepatocytes. Columns represent biological replicates at passages ranging from passage 1 - 40.

Figure 5. Expansion of Organoids grown in HepatiCult™ Organoid Growth Medium (Mouse)

Organoids cultured with HepatiCult™ Organoid Growth Medium (Mouse) show efficient growth over passaging. Cultures were split with an average split ratio of 1:25 at each passage.

Figure 6. Differentiation of Hepatic Progenitor Organoids

Hepatic progenitor organoids grown in HepatiCult™ Organoid Growth Medium (Mouse) (EM) can be differentiated to resemble more mature cell types when switched to a differentiation medium (DM)1,2. After switching to published differentiation medium hepatic organoids show the upregulation of mature hepatic markers such as (A) Hnf4α and (B) Alb, downregulation of hepatic stem cell and progenitor markers (C) Sox9 and (D) Axin2 and upregulation of ductal markers (E) Krt19 and (F) Hnf1β. Relative quantification (RQ) for each marker is reported relative to 18S and TBP housekeeping genes and normalized relative to hepatic progenitor organoids grown in HepatiCult™ Organoid Growth Medium (Mouse) and cultured in Matrigel® domes.

Publications (1)

Cell death {\&} disease 2019 Modulation of the extrinsic cell death signaling pathway by viral Flip induces acute-death mediated liver failure. M. Bittel et al.

Abstract

During viral infections viruses express molecules that interfere with the host-cell death machinery and thus inhibit cell death responses. For example the viral FLIP (vFLIP) encoded by Kaposi's sarcoma-associated herpesvirus interacts and inhibits the central cell death effector, Caspase-8. In order to analyze the impact of anti-apoptotic viral proteins, like vFlip, on liver physiology in vivo, mice expressing vFlip constitutively in hepatocytes (vFlipAlbCre+) were generated. Transgenic expression of vFlip caused severe liver tissue injury accompanied by massive hepatocellular necrosis and inflammation that finally culminated in early postnatal death of mice. On a molecular level, hepatocellular death was mediated by RIPK1-MLKL necroptosis driven by an autocrine TNF production. The loss of hepatocytes was accompanied by impaired bile acid production and disruption of the bile duct structure with impact on the liver-gut axis. Notably, embryonic development and tissue homeostasis were unaffected by vFlip expression. In summary our data uncovered that transgenic expression of vFlip can cause severe liver injury in mice, culminating in multiple organ insufficiency and death. These results demonstrate that viral cell death regulatory molecules exhibit different facets of activities beyond the inhibition of cell death that may merit more sophisticated in vitro and in vivo analysis.
View All Publications

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