STEMdiff™ Midbrain Neuron Maturation Kit

Maturation kit for generation of functional midbrain-type neurons from human ES and iPS cell-derived neuronal precursor cells
STEMdiff™ Midbrain Neuron Maturation Kit

Maturation kit for generation of functional midbrain-type neurons from human ES and iPS cell-derived neuronal precursor cells

1 Kit
Catalog # 100-0041
310 USD
Required Products
  1. ACCUTASE™
    ACCUTASE™

    Cell detachment solution

  2. STEMdiff™ SMADi Neural Induction Kit
    STEMdiff™ SMADi Neural Induction Kit

    Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells

  3. STEMdiff™ Midbrain Neuron Differentiation Kit
    STEMdiff™ Midbrain Neuron Differentiation Kit

    Differentiation kit for the generation of midbrain-patterned neuronal precursors from human ES and iPS cell-derived neural progenitor cells

Overview

The STEMdiff™ Midbrain Neuron Maturation Kit (Catalog #100-0041) is used in conjunction with the STEMdiff™ Midbrain Neuron Differentiation Kit (Catalog #100-0038) to generate a mixed population of midbrain-type neurons from neural progenitor cells derived from human pluripotent stem cells. These media will produce a population of midbrain neurons (≥ 15% TH-positive dopaminergic neurons; ≥ 90% class III βtubulin-positive neurons; < 10% GFAP-positive astrocytes). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.
Advantages
• Defined and serum-free
• Supports highly efficient generation of midbrain-like functional neurons from hPSC-derived neuronal precursors
• Produces a population of TH-positive neurons (>15%) that can be maintained long term in culture
• Optimized for maturation of midbrain neuronal precursors generated using the STEMdiff™ Midbrain Neuron Differentiation Kit
• Supports neuronal activity for physiologically relevant results
• Enables reproducible maturation of midbrain-type neuronal precursors derived from multiple human ES and iPS cell lines
Components
  • STEMdiff™ Midbrain Neuron Maturation Supplement, 25 mL
  • BrainPhys™ Neuronal Medium, 100 mL
Subtype
Specialized Media
Cell Type
Dopaminergic Neurons, Neural Cells, PSC-Derived, Neurons
Species
Human
Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunocytochemistry, Phenotyping
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
Formulation
Serum-Free, Chemically Defined

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Midbrain Neuron Maturation Kit
Catalog #
100-0041
Lot #
All
Language
English

Educational Materials (8)

Brochure
STEMCELL Neural Product Portfolio
Brochure
hPSC-Derived Neural Cell Research
Brochure
Products for Human Pluripotent Stem Cells
Wallchart
Cell-Reprogramming Technology and Neuroscience
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Neural Stem Cells
Webinar
The Road to Functional Human Neuronal Circuits in Vitro
56:27
The Road to Functional Human Neuronal Circuits in Vitro
Webinar
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
24:46
Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 12 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 6 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

Figure 4. Midbrain-Type Neural Precursor Cells Express Characteristic Markers After Culture in STEMdiff™ Midbrain Neuron Differentiation Kit

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

Figure 5. Midbrain-Type Neurons Express Dopamine Transporters (DAT) After Differentiation and Maturation in STEMdiff™ Midbrain Neuron Kits

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).

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