STEMdiff™ Endothelial Expansion Medium Kit
Expand human pluripotent stem cell-derived endothelial cells
STEMdiff™ Endothelial Expansion Medium Kit
Expand human pluripotent stem cell-derived endothelial cells
What's Included
- STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007)
- STEMdiff™ Endothelial Expansion Basal Medium, 120 mL
- STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL
-
Heparin SolutionCell culture supplement
-
Animal Component-Free Cell Dissociation KitDissociation kit for human stem and progenitor cells
Overview
NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.
CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Data Figures
Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit
In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).
Figure 2. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium
STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (2)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
