STEMdiff™ Endothelial Expansion Medium Kit

Expand human pluripotent stem cell-derived endothelial cells
STEMdiff™ Endothelial Expansion Medium Kit

FBS-free medium for the expansion of human pluripotent stem cell-derived endothelial cells

1 Kit
Catalog # 08007
293 USD

Overview

STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007) includes the xeno-free endothelial expansion basal medium and supplement, and must be used in conjunction with Animal Component-Free Cell Attachment Substrate. STEMdiff™ Endothelial Expansion Medium is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.

NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.

CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate.
Components
  • STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007)
    • STEMdiff™ Endothelial Expansion Basal Medium, 120 mL
    • STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL
Contains
Cell Culture Media and Supplements
Subtype
Specialized Media
Cell Type
Endothelial Cells
Species
Human
Application
Cell Culture, Expansion
Brand
STEMdiff
Area of Interest
Disease Modeling, Endothelial Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Endothelial Expansion Medium Kit
Catalog #
08007
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Endothelial Expansion Medium Kit
Catalog #
08007
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Endothelial Expansion Medium Kit
Catalog #
08007
Lot #
All
Language
English

Educational Materials (1)

Scientific Poster
STEMdiff™ Endothelial: A New Culture Workflow for Efficient Derivation and Expansion of Endothelial Cells From Human Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit

In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).

Figure 2. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium

STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.