STEMdiff™ Endothelial Expansion Medium Kit
Expand human pluripotent stem cell-derived endothelial cells
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STEMdiff™ Endothelial Expansion Medium Kit -
Label for STEMdiff™ Endothelial Expansion Medium Kit -
Label for STEMdiff™ Endothelial Expansion Medium Kit
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Required Products
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Animal Component-Free Cell Dissociation Kit
Dissociation kit for human stem and progenitor cells -
Cell culture supplement
Overview
STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007) includes the xeno-free endothelial expansion basal medium and supplement, and must be used in conjunction with Animal Component-Free Cell Attachment Substrate. STEMdiff™ Endothelial Expansion Medium is optimized for the expansion of hPSC-derived endothelial cells and for the maintenance of endothelial marker expression. For passaging, Animal Component-Free Cell Dissociation Kit (Catalog #05426) is required.
NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.
NOTE: Heparin Solution (Catalog #07980) is required for preparation of complete STEMdiff™ Endothelial Expansion Medium.
Components:
- STEMdiff™ Endothelial Expansion Medium Kit (Catalog #08007)
- STEMdiff™ Endothelial Expansion Basal Medium, 120 mL
- STEMdiff™ Endothelial Expansion 5X Supplement, 30 mL
Contains:
Cell Culture Media and Supplements
Subtype:
Specialized Media
Cell Type:
Endothelial Cells
Species:
Human
Application:
Cell Culture; Expansion
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Endothelial Cell Biology
Formulation:
Serum-Free
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
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Educational Materials
(1)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit
In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).
Figure 2. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium
STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.