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ReproTeSR™

Reprogramming medium for human iPS cell induction

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ReproTeSR™

Reprogramming medium for human iPS cell induction

500 mL
Catalog #05920
234 USD

Overview

ReproTeSR™ is a complete, defined, serum-free and xeno-free reprogramming medium. This medium is used during the generation of iPS cells from somatic cells, such as fibroblasts and other cell types, under feeder-free conditions.

ReproTeSR™ is also available as part of an integrated set of tools that are optimized for reprogramming blood-derived cell types expanded in vitro from peripheral blood, such as CD34+ cells (CD34+ Progenitor Reprogramming Kit, Catalog #05925) or erythroid cells (Erythroid Progenitor Reprogramming Kit, Catalog #05924).
Advantages:
• Defined, feeder-free formulation facilitates reproducibly efficient human iPS cell generation

• Rapid emergence of large colonies with high quality iPS cell-like morphology facilitates identification and subcloning for easily establishing iPS cell lines

• Seamlessly integrates with STEMCELL products prior to reprogramming and after iPS cell generation for maintenance and differentiation
Components:
  • ReproTeSR™ Basal Medium, 474 mL
  • ReproTeSR™ 20X Supplement, 25 mL
  • ReproTeSR™ 500X Supplement, 1 mL
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells; Hematopoietic Stem and Progenitor Cells
Species:
Human
Application:
Reprogramming; Cell Culture
Brand:
TeSR
Area of Interest:
Stem Cell Biology
Formulation:
Serum-Free; Xeno-Free; Defined

Scientific Resources

Product Documentation

Educational Materials

(5)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Figure 1. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean +/- SEM; erythroid cells, n=4; CD34+ cells, n=5.

ReproTeSR™ Efficiently Reprograms Fibroblasts

Figure 2. ReproTeSR™ Efficiently Reprograms Fibroblasts

Dermal fibroblasts were transfected with the ReproRNA™-OKSGM vector and reprogrammed under feeder-dependent (standard KOSR-containing hES cell medium on inactivated mouse embryonic fibroblasts (iMEFs)) or feeder-independent conditions (ReproTeSR™ on Corning® Matrigel®). Fibroblasts (passage 4) were reprogrammed with average efficiencies of 0.10 ± 0.06% (hES cell medium) and 0.20 ± 0.07% (ReproTeSR™). Reprogramming efficiency of fibroblasts with ReproRNA™ and ReproTeSR™ is comparable to that reported with Sendai virus. (Schlaeger TM, et al. (2015) Nat Biotechnol 33(1): 58-63.) (n ≥ 6; Data shown are mean ± SD).

Feeder-Free Reprogramming with ReproRNA™-OKSGM Vector and ReproTeSR™ Generates iPS Cell Colonies with Superior Colony Morphology

Figure 3. Feeder-Free Reprogramming with ReproRNA™-OKSGM Vector and ReproTeSR™ Generates iPS Cell Colonies with Superior Colony Morphology

Representative images of iPS cell colonies were generated using ReproRNA™‑OKSGM and cultured in (A) standard hES cell medium on irradiated mouse embryonic fibroblasts (iMEFs) or (B) ReproTeSR™ on Corning® Matrigel®. iPS cell colonies derived using ReproTeSR™ exhibit more defined borders, compact morphology, and reduced differentiation as compared to the ES cell medium.

Publications

(3)
Journal of the American Society of Nephrology : JASN 2011 JUL

Generation of induced pluripotent stem cells from urine

Zhou T et al.

Abstract

Forced expression of selected transcription factors can transform somatic cells into embryonic stem cell (ESC)-like cells, termed induced pluripotent stem cells (iPSCs). There is no consensus regarding the preferred tissue from which to harvest donor cells for reprogramming into iPSCs, and some donor cell types may be more prone than others to accumulation of epigenetic imprints and somatic cell mutations. Here, we present a simple, reproducible, noninvasive method for generating human iPSCs from renal tubular cells present in urine. This procedure eliminates many problems associated with other protocols, and the resulting iPSCs display an excellent ability to differentiate. These data suggest that urine may be a preferred source for generating iPSCs.
Cell stem cell 2010 JUL

Reprogramming of T cells from human peripheral blood.

Loh Y-H et al.

Abstract

Vogt-Koyanagi-Harada (VKH) disease (and sympathetic ophthalmia) is an ocular inflammatory disease that is considered to be a cell-mediated autoimmune disease against melanocytes. The purpose of this study was to determine the Ags specific to VKH disease and to develop an animal model of VKH disease. We found that exposure of lymphocytes from patients with VKH disease to peptides (30-mer) derived from the tyrosinase family proteins led to significant proliferation of the lymphocytes. Immunization of these peptides into pigmented rats induced ocular and extraocular changes that highly resembled human VKH disease, and we suggest that an experimental VKH disease was induced in these rats. We conclude that VKH disease is an autoimmune disease against the tyrosinase family proteins.
Yearbook of Dermatology and Dermatologic Surgery 2008 DEC

Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells

Yu J et al.

Abstract

Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.