CD34+ Progenitor Reprogramming Kit

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells
CD34+ Progenitor Reprogramming Kit

Integrated workflow containing tools to isolate and expand CD34+ progenitor cells from peripheral blood and their subsequent reprogramming to iPS cells

1 Kit
Catalog # 05925
1,849 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The CD34+ Progenitor Reprogramming Kit contains an integrated set of tools and reagents to enrich, expand and reprogram CD34+ progenitor cells from human peripheral blood samples.
Advantages
• Optimized for enrichment, expansion and reprogramming of CD34+ progenitor cells expanded from peripheral blood samples
• Improved reprogramming efficiency and higher frequency of iPS cell colonies, compared to traditional hES cell medium
• Rapid emergence of large colonies with high quality iPS cell-like morphology facilitates identification and subcloning
• Seamlessly integrates with TeSR™ and STEMdiff™ products for downstream maintenance and differentiation of iPS cell lines
Components
  • SepMate™-50 (Catalog #15440)
  • Lymphoprep™ (Catalog #07801)
  • Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086)
    • RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2 mL
    • EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
  • StemSpan™ SFEM II (Catalog #09605)
  • StemSpan™ CD34+ Expansion Supplement (Catalog #02691)
  • ReproTeSR™ (Catalog #05926)
    • TeSR™-E7™/ReproTeSR™ Basal Medium
    • ReproTeSR™ 25X Supplement
Subtype
Specialized Media
Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Reprogramming
Brand
StemSpan, TeSR
Area of Interest
Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Manual
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
CD34+ Progenitor Reprogramming Kit
Catalog #
05925
Lot #
All
Language
English

Educational Materials (4)

Brochure
An Integrated Workflow for Reprogramming Blood Cells
Brochure
Products for Human Pluripotent Stem Cells
Brochure
2019-2020 Cell Culture Training Catalog
Scientific Poster
Defined and Xeno-Free Medium for Reprogramming Blood-Derived CD34+ Cells or Erythroid Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

Figure 1. Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells

(A) In whole PB, the proportion of CD34+/CD45+ cells is typically very low (<1%). (B) In the RosetteSep™ enrichment step, unwanted cells are removed during density gradient centrifugation. This step enriches the population of CD34+/CD45+ cells (19.8%) in this example). (C) After positive selection of CD34+ cells using EasySep™, a highly pure population of CD34+/CD45+ cells (96.2% in this example) can be obtained. Values are reported as a percentage of viable CD45+ cells.

StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

Figure 2. StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells

TNC: Total Nucleated Cell

Schematic of ReproTeSR™ Reprogramming Timeline

Figure 3. Schematic of ReproTeSR™ Reprogramming Timeline

ReproTeSR™ is used during the entire induction phase of reprogramming (day 3 to 21). On days 3 and 5, ReproTeSR™ is added to StemSpan™ growth media (in a fed-batch manner) to facilitate attachment of transfected cells. Attached cells are further cultured in ReproTeSR™ with daily full media changes until putative iPS cell colonies emerge (days 21-28). iPS cell colonies can then be isolated and propagated in TeSR™ media. (mTeSR™1, TeSR™2, TeSR™-E8™).

Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Figure 4. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium

Efficiency of reprogramming (A) erythroid cells, or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean +/- SEM, erythroid cells n=4, CD34+ cells n=5.

ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Figure 5. ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology

Representative images of iPS cell colonies generated from isolated CD34+ progenitor cells using (A) ReproTeSR™ and (B) hESC) medium. iPS cell colonies produced using ReproTeSR™ exhibit more defined borders, compact morphology and reduced differentiation compared with hESC medium. 200X magnification.

Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Figure 6. Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™

Average values in bold (range).

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