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ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing

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ArciTect™ Human HPRT Positive Control Kit

Positive control for CRISPR-Cas9 genome editing

1 Kit
Catalog #76013
149 USD

Required Products

Overview

ArciTect™ Human HPRT Positive Control Kit is designed as a positive control for experiments using the ArciTect™ CRISPR-Cas9 genome editing system. The kit comprises ArciTect™ Human HPRT crRNA (2 nmol) and ArciTect™ Human HPRT Primer Mix (2 nmol), both of which have been tested and validated for use with the ArciTect™ line of genome editing products. HPRT, or hypoxanthine phosphoribosyltransferase, is a housekeeping gene and a commonly used control. The kit can be used to optimize transfection protocols and act as a positive control that can be used alongside custom ArciTect™ crRNAs (e.g. Catalog #76010). ArciTect™ Human HPRT crRNA first requires annealing to ArciTect™ tracrRNA (Catalog #76016) then must be combined with an ArciTect™ Cas9 Nuclease (e.g. Catalog #76002) to form a ribonucleoprotein complex. ArciTect™ Human HPRT Primer Mix can be used to amplify genomic DNA isolated from a population of transfected cells, which can subsequently be used in a T7 endonuclease I assay to determine cleavage genome editing efficiency.
Components:
  • ArciTect™ Human HPRT crRNA, 2 nmol
  • ArciTect™ Human HPRT Primer Mix, 2 nmol
Cell Type:
Pluripotent Stem Cells
Species:
Human
Brand:
ArciTect
Area of Interest:
Stem Cell Biology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.