Human Recombinant IL-10, ACF

Interleukin 10, animal component-free
Catalog #
78144_C
Interleukin 10, animal component-free
From: 1,117 USD

Overview

Interleukin 10 (IL-10) is the founding member of the IL-10 family of class II cytokines. All of the IL-10 cytokine family members have a four-helix bundle consisting of α-helical folds. Upon binding to its receptor, IL-10 activates signaling through JAK1 and STAT3. It is produced by dendritic cells, macrophages, and CD4 T cells, as well as mast cells, NK cells, neutrophils, and B cells, under specific stimulating conditions (Saraiva & O'Garra). IL-10 can inhibit the expression of pro-inflammatory cytokines and promote healing processes, and is important for the function of T regulatory cells. IL-10 also enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression, while IL-10 produced by macrophages inhibits activation of neighboring macrophages, thus allowing a level of self-regulation (Ouyang et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
B-TCGF, CSIF, Cytokine synthesis inhibitory factor, Interleukin-10, TGIF
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Myeloid Cells, Neurons, T Cells
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
18.8 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-10, ACF
Catalog #
78144.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-10, ACF
Catalog #
78144.1
Lot #
All
Language
English

Educational Materials (5)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-10, ACF was tested by its ability to promote the proliferation of MC/9 cells in the presence of human IL-4. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.95 ng/mL. (B) 1 μg of Human Recombinant IL-10, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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