Human Recombinant IL-10, ACF

Interleukin 10, animal component-free

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Interleukin 10, animal component-free
From: 274 USD

Overview

Interleukin 10 (IL-10) is the founding member of the IL-10 family of class II cytokines. All of the IL-10 cytokine family members have a four-helix bundle consisting of α-helical folds. Upon binding to its receptor, IL-10 activates signaling through JAK1 and STAT3. It is produced by dendritic cells, macrophages, and CD4 T cells, as well as mast cells, NK cells, neutrophils, and B cells, under specific stimulating conditions (Saraiva & O'Garra). IL-10 can inhibit the expression of pro-inflammatory cytokines and promote healing processes, and is important for the function of T regulatory cells. IL-10 also enhances B cell proliferation, immunoglobulin secretion, and class II MHC expression, while IL-10 produced by macrophages inhibits activation of neighboring macrophages, thus allowing a level of self-regulation (Ouyang et al.). This product is animal component-free.
Alternative Names:
B-TCGF, CSIF, Cytokine synthesis inhibitory factor, Interleukin-10, TGIF
Cell Type:
Dendritic Cells; Hematopoietic Stem and Progenitor Cells; Myeloid Cells; Neurons; T Cells
Area of Interest:
Immunology; Neuroscience; Stem Cell Biology
Molecular Weight:
18.8 kDa
Purity:
≥ 95%

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Data and Publications

Data

(A) The biological activity of Human Recombinant IL-10, ACF was tested by its ability to promote the proliferation of MC/9 cells in the presence of human IL-4. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.95 ng/mL. (B) 1 μg of Human Recombinant IL-10, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.