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Human Recombinant GM-CSF (E. coli-expressed)

Granulocyte-macrophage colony-stimulating factor

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Granulocyte-macrophage colony-stimulating factor
From: 190 USD

Overview

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilization of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).
Alternative Names:
Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoitin-alpha
Cell Type:
Hematopoietic Stem and Progenitor Cells; Dendritic Cells; Monocytes; Myeloid Cells; Mesoderm, PSC-Derived
Species:
Human
Area of Interest:
Stem Cell Biology; Immunology
Molecular Weight:
14.6 kDa
Purity:
≥ 95%

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(3)

Data and Publications

Data

(A) The biological activity of Human Recombinant GM-CSF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.08 - 0.12 ng/mL.
(B) 1 μg of Human Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GM-CSF has a predicted molecular mass of 14.6 kDa.

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