Human Recombinant BDNF, ACF

Brain-derived neurotrophic factor, animal component-free
Catalog #
78133_C
Brain-derived neurotrophic factor, animal component-free
From: 204 USD

Overview

Brain-derived neurotrophic factor (BDNF), like nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4), is a member of the NGF family of neurotrophins, which are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems (Minichiello & Klein; Minichiello et al.). BDNF binds with high affinity to the TRKB kinase receptor, and activates AKT and ERK pathways (Mattson et al.). It is expressed in hippocampus, cortex, and synapses of the basal forebrain. BDNF acts as a survival factor for human embryonic stem cells when plated on either feeder cells or Corning® Matrigel® (Pyle et al.). BDNF regulates synaptic transmission and plasticity at adult synapses in the central nervous system, contributes to adaptive neuronal responses including long-term potentiation, long-term depression, certain forms of short-term synaptic plasticity, as well as homeostatic regulation of neuronal excitability (Reichardt). It also has a role in neurogenesis by promoting survival and growth of dorsal root ganglion cells, and hippocampal and cortical neurons (Binder & Scharfman). BDNF, together with glial cell-derived neurotrophic factor (GDNF) and other supplements, is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Brafman). This product is animal component-free.
Subtype
Cytokines
Alternative Names
Abrineurin, ANON2, BULN2, Neurotrophin, MGC34632
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
13.6 kDa monomer; 27.3 kDa dimer
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant BDNF, ACF
Catalog #
78133, 78133.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant BDNF, ACF
Catalog #
78133, 78133.1
Lot #
All
Language
English

Educational Materials(3)

Brochure
BrainPhys™ Neuronal Medium for Improved Neuronal Function
Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Small Molecules for Stem Cell Research

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant BDNF, ACF was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6). Cell proliferation was measured using a colorimetric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 5 ng/mL. For a representative bioactivity plot of C6 cell proliferation, contact us at techsupport@stemcell.com.
(B) 1 μg of Human Recombinant BDNF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant BDNF, ACF is a homodimer of 13.6 kDa subunits with a predicted molecular mass of 27.3 kDa.

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