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EasySep™ Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection cell enrichment kit

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From: 799 USD

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Immunomagnetic negative selection cell enrichment kit
From: 799 USD

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Overview

The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
Advantages:
• Fast, easy-to-use and column-free
• Untouched, viable cells
• Facilitates rapid flow sorting of ILCs
Components:
  • EasySep™ Human Pan-ILC Enrichment Kit (Catalog #17975)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
 
  • RoboSep™ Human Pan-ILC Enrichment Kit (Catalog #17975RF)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
Innate Lymphoid Cells
Species:
Human
Sample Source:
Leukapheresis; PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Typical EasySep™ Human Pan-ILC Enrichment Profile

Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.