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The 6H6 antibody reacts with human CD123 (IL-3 receptor subunit α), an ~70 kDa type I transmembrane glycoprotein belonging to the type I cytokine receptor family (type 5 subfamily) and the immunoglobulin (Ig) superfamily. CD123 constitutes the ligand-binding α chain of the heterodimeric IL-3 receptor. CD123 binds IL-3 with low affinity per se, but when associated with CD131 (the signal-transducing β chain of the receptor), high-affinity binding of IL-3 is observed. CD123 is expressed by hematopoietic progenitor cells, endothelial cells, basophils, eosinophils, mast cells, monocytes, macrophages, dendritic cells, megakaryocytes, a subset of B cells, and by neutrophils if cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-3 binding to CD123 stimulates proliferation, differentiation, and viability of hematopoietic cells. CD123 is highly expressed in malignancies such as acute leukemia. The 6H6 antibody does not inhibit binding of IL-3 to either CD123 or the IL-3 receptor.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60071) followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, Biotin followed by streptavidin (SAV) APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60071BT) followed by SAV APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, FITC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (C) Flow cytometry analysis (gated on CD45+) of human whole blood nucleated cells processed with the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) and labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and an anti-IgE antibody, FITC. Labeling of the Start cells prior to cell isolation is shown (D).
Figure 6. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PerCP-Cy5.5 and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCPCy5.5 (Catalog #60070PS) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
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Mouse monoclonal IgG1, kappa isotype control antibody
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Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
