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The 104D2 antibody reacts with CD117 (c-Kit), an ~145 kDa type 1 transmembrane receptor for c-Kit ligand (stem cell factor/steel factor) that is broadly expressed on hematopoietic stem cells in bone marrow, including pluripotent and erythroid progenitor cells and B and T lymphocyte precursors, as well as on mast cells and melanocytes. CD117 is expressed on ~1 - 4% of bone marrow cells, the majority of which (~50 - 70%) co-express CD34 and comprise progenitor cells and their precursors. CD117 belongs to the tyrosine kinase receptor family and possesses five immunoglobulin-like C2-type domains and a cytoplasmic protein kinase domain. Binding of c-Kit ligand to CD117 induces dimerization and autophosphorylation, which activates several intracellular signaling pathways critical for the proliferation and differentiation of hematopoietic stem cells. Signaling by CD117 is modulated by phosphatases and by rapid endocytosis and degradation of the receptor. Binding of the 104D2 antibody does not block binding of c-Kit ligand or antibody clone SR1.
Subtype
Primary Antibodies
Target Antigen
CD117 (c-Kit)
Alternative Names
c-KIT, cKIT, stem cell factor receptor (SCFR)
Reactive Species
Cow, Cynomolgus, Human
Conjugation
APC, Biotin, PE, Unconjugated
Host Species
Mouse
Cell Type
Endoderm, PSC-Derived, Hematopoietic Stem and Progenitor Cells
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).
(B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).
(C) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (Catalog #60089PE). The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184.
(D) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE).
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).
Figure 4. Data for PE-Conjugated
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
This product is designed for use in the following research area(s) as part
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Mouse monoclonal IgG1, kappa isotype control antibody
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Anti-Human CD117 (c-Kit) Antibody, Clone 104D2
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
