Anti-Human CD117 (c-Kit) Antibody, Clone 104D2
Mouse monoclonal IgG1 antibody against human, cynomolgus, cow CD117 (c-Kit)
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Overview
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human induced pluripotent stem (iPS) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram). (C) Flow cytometry analysis of human embryonic stem (ES) cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, APC and Anti-Human CD184 (CXCR4) Antibody, Clone 12G5, PE (Catalog #60089PE). The emergence of definitive endoderm can be monitored by co-expression of CD117 and CD184. (D) Flow cytometry analysis of human ES cells following differentiation to definitive endoderm using the STEMdiff™ Definitive Endoderm Kit. Cells were labeled with a mouse IgG1, kappa APC isotype control antibody and Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE).
Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).
Figure 4. Data for PE-Conjugated
Flow cytometry analysis of human erythroleukemia (HEL) cells labeled with Anti-Human CD117 (c-Kit) Antibody, Clone 104D2, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (1)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
