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EasySep™ Human Naïve CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

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From: 778 USD

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Immunomagnetic negative selection kit
From: 778 USD

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Overview

The EasySep™ Human Naïve CD8+ T Cell Isolation Kit is designed to isolate naïve CD8+ T cells from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD4, CD14, CD16, CD19, CD20, CD36, CD45RO, CD56, CD57, CD94, CD123, CD244, TCRγ/δ, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

For even faster cell isolations, we recommend the EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (17968) which isolates cells in as little as 11 minutes.
Advantages:
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
Components:
  • EasySep™ Human Naïve CD8+ T Cell Isolation Kit (Catalog #19258)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D2 Magnetic Particles, 2 x 1 mL
  • RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #19258RF)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ D2 Magnetic Particles, 5 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) x 2
 
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD8+
Species:
Human
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data


Starting with fresh PBMCs, the naïve CD8+ T cell content (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) of the isolated fraction is typically 92.3 ± 4.0% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.9% and 92.8%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.