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EasySep™ Human Naïve CD8+ T Cell Isolation Kit II

Immunomagnetic negative selection kit

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From: 778 USD

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Immunomagnetic negative selection kit
From: 778 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human Naïve CD8+ T Cell Isolation Kit II is designed to isolate naïve CD8+ T cells from fresh peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically-labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages:
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
Components:
  •  EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (Catalog #17968)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 x 1 mL
  •  RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #17968RF)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125) 
 
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD8+; T Cells
Species:
Human
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(9)
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Scientific Poster
Isolation of Untouched Human Naive and Memory CD8 T Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
T Cell Engineering
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Cell Cryopreservation

Data and Publications

Data

Starting with fresh PBMCs, the naïve CD8+ T cell content (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) of the isolated fraction is typically 93.7 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.8% and 94.2%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.