EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Immunomagnetic negative selection kit
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EasySep™ Human Naïve CD8+ T Cell Isolation Kit II -
EasySep™ Human Naïve CD8+ T Cell Isolation Kit II -
Label for EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Human Naïve CD8+ T Cell Isolation Kit II is designed to isolate naïve CD8+ T cells from fresh peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically-labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19158) for even faster cell isolations.
This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19158) for even faster cell isolations.
Advantages:
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
• Up to 97% purity
• Untouched, viable cells
Components:
- EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (Catalog #17968)
- EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 x 1 mL
- RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #17968RF)
- EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™, 1 x 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD8+
Species:
Human
Sample Source:
PBMC
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Immunology
Related Products
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"The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation
Labeling Antibodies
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Anti-Human CD45RO Antibody, Clone UCHL1
Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO -
Anti-Human CD8a Antibody, Clone RPA-T8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a -
Anti-Human CD56 (NCAM) Antibody, Clone HCD56
Mouse monoclonal IgG1 antibody against human CD56 (NCAM)
Scientific Resources
Product Documentation
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(9)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with fresh PBMCs, the naïve CD8+ T cell content (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) of the isolated fraction is typically 93.7 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.8% and 94.2%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.