EasySep™ Human Naïve CD8+ T Cell Isolation Kit II

Immunomagnetic negative selection kit
Catalog #
17968_C
Immunomagnetic negative selection kit
From: 858 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human Naïve CD8+ T Cell Isolation Kit II is designed to isolate naïve CD8+ T cells from fresh peripheral blood mononuclear cells by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically-labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.

This product replaces the EasySep™ Human Naïve CD8+ T Cell Enrichment Kit (Catalog #19158) for even faster cell isolations.
Advantages
• Fast, easy-to-use and column-free
• Up to 97% purity
• Untouched, viable cells
Components
  • EasySep™ Human Naïve CD8+ T Cell Isolation Kit II (Catalog #17968)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 x 1 mL
  • RoboSep™ Human Naive CD8+ T Cell Isolation Kit (Catalog #17968RF)
    • EasySep™ Human Naïve CD8+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™, 1 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Related Products

Labeling Antibodies

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human Naïve CD8+ T Cell Isolation Kit II
Catalog #
17968RF
Lot #
All
Language
English

Educational Materials(9)

Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Antigen Processing and Presentation
Wallchart
Human Immune Cytokines
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Scientific Poster
Isolation of Untouched Human Naive and Memory CD8 T Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with fresh PBMCs, the naïve CD8+ T cell content (CD8+CD45RA+CCR7+ and CD45RO-CD57-CD56-) of the isolated fraction is typically 93.7 ± 2.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 6.8% and 94.2%, respectively.

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