EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit

Immunomagnetic positive selection cell isolation kit

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EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit

Immunomagnetic depletion of human CD25+ cells

1 x 109 cells
Catalog #17861
667 USD

Required Products

Overview

The EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit is designed to deplete CD25+ cells from fresh peripheral blood mononuclear cells or lysed leukapheresis samples. CD25+ cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Labeled cells remain in the tube while unlabeled cells are poured off. The CD25 antigen is expressed on regulatory T cells and activated T and B cells.
Advantages:
• Fast, easy-to-use and column-free
• Up to 1.3 log depletion of CD25+ cells
• Up to 98% purity
Components:
EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit (Catalog #17861)
    • EasySep™  Human Pan-CD25 Positive Selection and Depletion Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells; T Cells, CD4+; T Cells, Regulatory
Species:
Human
Sample Source:
PBMC
Selection Method:
Depletion
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(9)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

CD25 cell depletion results using EasySep™

Starting with fresh PBMCs, the CD25+ cell content of the depleted fraction typically ranges from 2 - 5%. In the above example, the purities of the start and final depleted fractions are 14.5% and 2.2%, respectively, with a 1.3 log depletion of CD25+ cells. Using the optional positive selection protocol, the CD25+ cell content of the isolated fraction typically ranges from 81 - 98% (data not shown).

Publications

(1)
Scientific reports 2017 AUG

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Albert BJ et al.

Abstract

Current antiretroviral therapy (ART) for HIV/AIDS slows disease progression by reducing viral loads and increasing CD4 counts. Yet ART is not curative due to the persistence of CD4+ T-cell proviral reservoirs that chronically resupply active virus. Elimination of these reservoirs through the administration of synergistic combinations of latency reversing agents (LRAs), such as histone deacetylase (HDAC) inhibitors and protein kinase C (PKC) modulators, provides a promising strategy to reduce if not eradicate the viral reservoir. Here, we demonstrate that largazole and its analogues are isoform-targeted histone deacetylase inhibitors and potent LRAs. Significantly, these isoform-targeted HDAC inhibitors synergize with PKC modulators, namely bryostatin-1 analogues (bryologs). Implementation of this unprecedented LRA combination induces HIV-1 reactivation to unparalleled levels and avoids global T-cell activation within resting CD4+ T-cells.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.