EasySep Human CD2 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
Catalog #
17883_C
Immunomagnetic positive selection cell isolation kit
From: 700 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer

Overview

The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.

This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
Advantages
• Fast and easy-to-use
• Up to 97% purity
• No columns required
Components
  • EasySep™ Human CD2 Positive Selection Kit (Catalog #17883)
    • EasySep™ Human CD2 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
  • RoboSep™ Human CD2 Positive Selection Kit (Catalog #17883RF)
    • EasySep™ Human CD2 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD2 Positive Selection Kit II
Catalog #
17883
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human CD2 Positive Selection Kit II
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD2 Positive Selection Kit II
Catalog #
17883
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD2 Positive Selection Kit II
Catalog #
17883
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Human CD2 Positive Selection Kit II
Catalog #
17883
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD2 Positive Selection Kit II
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD2 Positive Selection Kit II
Catalog #
17883RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD2 Positive Selection Kit II
Catalog #
17883RF
Lot #
All
Language
English

Educational Materials (10)

Brochure
EasySep™ Cell Separation Technology
Brochure
Isolate Human Immune Cells
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
The Immune Response to HIV Poster
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Video
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Starting with PBMCs, the CD2+ cell content of the isolated fraction is typically 93.8 ± 3.3% (mean ± SD using the purple EasySep™ Magnet; as assessed by labeling with CD3 and CD56). In the above example, the purities of the start and final isolated fractions are 66.1% and 94.8%, respectively.

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