EasySep™ Dead Cell Removal (Annexin V) Kit
Immunomagnetic depletion cell isolation kit
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EasySep™ Dead Cell Removal (Annexin V) Kit -
Label for EasySep™ Dead Cell Removal (Annexin V) Kit
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Required Products
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Magnet for column-free immunomagnetic separation
Overview
The EasySep™ Dead Cell Removal (Annexin V) kit is designed to deplete apoptotic (Annexin V+) cells from cell culture or tissue preparations by immunomagnetic negative selection. Unwanted cells are labeled with Annexin V, antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are simply poured off into a new tube. Annexin V binds phosphatidylserine on the outer leaflet of the cell membrane during apoptosis.
Advantages:
• Fast and easy-to-use
• No columns required
• Compatible across EasySep™, "The Big Easy", and EasyEights™ platforms
• No columns required
• Compatible across EasySep™, "The Big Easy", and EasyEights™ platforms
Components:
- EasySep™ Dead Cell Removal (Annexin V) Kit (Catalog #17899)
- EasySep™ Dead Cell Removal (Annexin V) Cocktail, 0.5 mL
- EasySep ™ Biotin Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50103, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000), or
• “The Big Easy” EasySep™ Magnet (Catalog #18001), or
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Lymphocytes
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Sample Source:
Cord Blood; Leukapheresis; Lung; Lymph Node; Other; Spleen
Selection Method:
Depletion
Brand:
EasySep
Area of Interest:
Immunology
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EasySep™ Mouse CD11c Positive Selection Kit II
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EasySep™ Mouse CD4+ T Cell Isolation Kit
15-Minute cell isolation kit using immunomagnetic negative selection
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with human polymorphonuclear cells (PMNCs) cultured overnight, the live cell content (AnnexinV-/PI-) of the enriched fraction is typically 69.7± 12.5% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of live cells in the start and final enriched fractions are 12.8% and 74.9%, respectively.
Starting with 24- to 48-hour-old mouse splenocytes, the live cell content of the enriched fraction is typically 79.8 ± 11.4% (mean ± SD using the purple EasySep™ Magnet). In the above example, the percentages of live cells in the start and final enriched fractions are 78.1% and 93.4%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.