Annexin V Apoptosis Detection Kit with 7-AAD

Apoptosis staining and detection kit
Catalog #
100-0337_C
Apoptosis staining and detection kit
From: 255 USD

Overview

The Annexin V Apoptosis Detection Kit includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001). The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both Annexin V and 7-AAD).
Annexin V belongs to the annexin family of intracellular proteins that bind to membrane phospholipids in a calcium-dependent manner. This dye has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the cell membrane, exposing it to the external environment, where it is detected by Annexin V. This process of PS translocation occurs prior to the loss of membrane integrity. As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD.
Components
  • Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333),1X 100 Tests
    • Annexin V Binding Buffer (Catalog #100-0334), 1 x 50 mL
    • 7-AAD (Catalog #75001), 1X 200 Tests
Conjugation
APC, FITC, PE
Application
Flow Cytometry
Area of Interest
Drug Discovery and Toxicity Testing, Immunology

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Annexin V Apoptosis Detection Kit with 7-AAD, PE
Catalog #
100-0337
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Annexin V Apoptosis Detection Kit with 7-AAD, FITC
Catalog #
100-0338
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Annexin V Apoptosis Detection Kit with 7-AAD, APC
Catalog #
100-0339
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD.

Figure 1. Data for Annexin V, APC

Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC-conjugated Annexin V and 7-AAD.

Figure 2. Data for Annexin V, FITC

Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC-conjugated Annexin V and 7-AAD (Catalog #75001).

Figure showing flow cytometry analysis of Con A-stimulated (3 days) C57BL/6 mouse splenocytes labeled with Anti-Mouse CD152 (CTLA-4) Antibody, Clone UC10-4F10-11, followed by a goat anti-Armenian hamster IgG antibody, FITC or an Armenian hamster IgG isotype control antibody, followed by a goat anti-Armenian hamster IgG, FITC.

Figure 3. Data for Annexin V, PE

Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE-conjugated Annexin V and 7-AAD.

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