STEMdiff™ Midbrain Neuron Maturation Kit

Maturation kit for generation of functional midbrain-type neurons from human ES and iPS cell-derived neuronal precursor cells

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STEMdiff™ Midbrain Neuron Maturation Kit

Maturation kit for generation of functional midbrain-type neurons from human ES and iPS cell-derived neuronal precursor cells

1 Kit
Catalog #100-0041
295 USD

Required Products

Overview

The STEMdiff™ Midbrain Neuron Maturation Kit (Catalog #100-0041) is used in conjunction with the STEMdiff™ Midbrain Neuron Differentiation Kit (Catalog #100-0038) to generate a mixed population of midbrain-type neurons from neural progenitor cells derived from human pluripotent stem cells. These media will produce a population of midbrain neurons (≥ 15% TH-positive dopaminergic neurons; ≥ 90% class III βtubulin-positive neurons; < 10% GFAP-positive astrocytes). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.
Advantages:
• Defined and serum-free
• Supports highly efficient generation of midbrain-like functional neurons from hPSC-derived neuronal precursors
• Produces a population of TH-positive neurons (>15%) that can be maintained long term in culture
• Optimized for maturation of midbrain neuronal precursors generated using the STEMdiff™ Midbrain Neuron Differentiation Kit
• Supports neuronal activity for physiologically relevant results
• Enables reproducible maturation of midbrain-type neuronal precursors derived from multiple human ES and iPS cell lines
Components:
  • STEMdiff™ Midbrain Neuron Maturation Supplement, 25 mL
  • BrainPhys™ Neuronal Medium, 100 mL
Subtype:
Specialized Media
Cell Type:
Dopaminergic Neurons; Neural Cells, PSC-Derived; Neurons
Species:
Human
Application:
Cell Culture; Characterization; Differentiation; Functional Assay; Immunocytochemistry; Phenotyping
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience
Formulation:
Serum-Free; Chemically Defined

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Embryoid Body Protocol)

Figure 1. Schematic for the Embryoid Body Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 12 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Experimental Protocol for STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits (Monolayer Protocol)

Figure 2. Schematic for the Monolayer Protocol

Midbrain-type neurons, including dopaminergic neurons, can be generated from neural precursors after 6 days in STEMdiff™ Midbrain Neuron Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.

Midbrain-Type Neurons Arise From Neural Progenitor Cells After Culture in STEMdiff™ SMADi Neural Induction Kit and STEMdiff™ Midbrain Culture System

Figure 3. Midbrain-Type Neurons Are Generated After Culture in STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.