Anti-Human CD45 Antibody, Clone 2D1

Mouse monoclonal IgG1 antibody against human CD45

Anti-Human CD45 Antibody, Clone 2D1

Mouse monoclonal IgG1 antibody against human CD45

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Mouse monoclonal IgG1 antibody against human CD45
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Overview

The 2D1 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of hematopoietic stem and progenitor cells and mature blood cells, except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell lineages. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains, one of which is enzymatically active, that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, and 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa.
Subtype
Primary Antibodies
Target Antigen
CD45
Reactive Species
Human
Conjugation
FITC
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Leukocytes, Lymphocytes, Myeloid Cells
Species
Human
Application
Cell Isolation, Flow Cytometry
Area of Interest
Immunology

Data Figures

FACS Profile Results With RosetteSep™ Human Cord Blood Progenitor CellEnrichment Kit

Figure 1. Data for FITC Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone 2D1, FITC (filled histogram) or Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60070FI; solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
60123FI.1, 60123FI
Lot #
All
Language
English
Catalog #
60123FI
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
60123FI.1, 60123FI
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications