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Anti-Mouse CD45R (B220) Antibody, Clone RA3-6B2

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220)
Catalog #
60019_C
Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220)
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Overview

The RA3-6B2 antibody reacts with an epitope on the extracellular domain of the exon A‐restricted isoform of mouse CD45. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of Src kinases such as Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 200 - 220 kDa. The exon A‐restricted isoform of mouse CD45, termed CD45R, contains the RA, RB, and RC regions and is the largest of the isoforms. CD45R is expressed by the B cell lineage from early pro‐B to mature B cells, with its expression downregulated during differentiation to plasma cells. CD45R is also expressed on subsets of T, NK, dendritic, and lymphokine-activated killer (LAK) cells.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse B Cell Isolation Kit (Catalog #19854).
Subtype
Primary Antibodies
Target Antigen
CD45R (B220)
Alternative Names
B220, Protein tyrosine phosphatase receptor type C, PTPRC
Reactive Species
Cat, Human, Mouse
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells, Leukocytes, Lymphocytes
Species
Human, Mouse, Other
Application
CyTOF, FACS, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology, Transplantation Research
Clone
RA3-6B2
Gene ID
19264/5788
Isotype
IgG2a, kappa

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Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
60019, 100-0422
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
60019BT, 100-0424, 100-0425
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
60019FI, 100-0423
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0420, 100-0421
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2
Catalog #
60019, 100-0422
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488
Catalog #
60019AD, 60019AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC
Catalog #
60019AZ, 60019AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin
Catalog #
60019BT, 100-0424, 100-0425
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC
Catalog #
60019FI, 100-0423
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5
Catalog #
60019PS, 60019PS.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™
Catalog #
60019PB, 60019PB.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE
Catalog #
100-0420, 100-0421
Lot #
All
Language
English

Educational Materials(4)

Brochure
Tools For Your Immunology Research
Brochure
Hematopoietic Stem and Progenitor Cells - Products for Your Research
Brochure
Reliable Antibodies for Your Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for B Cell Research
Cell Sourcing
Cell Isolation
Cell Expansion
Cell Characterization
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Cell Sourcing and Isolation
Expansion and Differentiation
Analysis
Workflow Stages for Total Lymphocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, followed by a mouse anti-rat IgG2a antibody, FITC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by a mouse anti-rat IgG2a antibody, FITC (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, FITC (Catalog #60076FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 5. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis fo C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, PerCP-Cy5.5 (filled histogram) or a rat IgG2a, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Data for Alexa Fluor® 488-Conjugated

Figure 6. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Alexa Fluor® 488 (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Alexa Fluor® 488 (Catalog #60076AD) (solid line histogram).

Data for Biotin-Conjugated

Figure 7. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD45R Antibody, Clone RA3-6B2, Pacific Blue™ (filled histogram) or a rat IgG2a, kappa Pacific Blue™ isotype control antibody (solid line histogram).

Legal Statement:

Alexa Fluor and Pacific Blue are trademarks of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® or Pacific Blue™ are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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