Overview
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Direct Human CD4+ T Cell Isolation Kit (Catalog #19662), EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052), EasySep™ Human CD3 Positive Selection Kit II (Catalog #17851) and EasySep™ Human CD4 Positive Selection Kit II (Catalog #17852).
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (Catalog #60072AD) is shown in the bottom panel (solid line histogram). (C) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD4 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 is shown in the bottom panel (solid line histogram).

Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, APC and Anti-Human CD45 Antibody, Clone HI30, FITC (Catalog #60018FI). (B) Flow cytometry analysis of human PBMCs labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (Catalog #60072AZ) and Anti-Human CD45 Antibody, Clone HI30, FITC.

Figure 3. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin (Catalog #60072BT), followed by SAV APC (solid line histogram).

Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, FITC and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (Catalog #60072FI) and Anti-Human CD45 Antibody, Clone HI30, APC.

Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, PE and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs isolated with the EasySep™ Human CD4 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, PE. Histograms show labeling of total PBMCs (Start) and isolated cells (Isolated). Labeling with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (Catalog #60072PE) is shown (solid line histogram).

Figure 6. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 (Catalog #60072), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Figure 7. Data for PerCP-Cy55-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD4 Antibody, Clone OKT4, PerCP-Cy5.5 (filled histogram), or Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (Catalog #60072PS; solid line histogram).