Anti-Human CD105 Antibody, Clone 43A3

Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin)
From: 105 USD

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Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin)
From: 105 USD

Overview

The 43A3 antibody reacts with CD105 (endoglin), an ~180 kDa cell surface glycoprotein which is a disulfide-bonded homodimer of ~90 kDa type I transmembrane subunits. CD105 is a component of the TGF-β receptor complex and is expressed by vascular endothelial smooth muscle cells, syncytiotrophoblasts of placenta and activated macrophages, and at relatively low levels by stromal fibroblasts. Its expression is also observed in some types of tumors, and levels are up-regulated on the endothelium during angiogenesis. In concert with signaling receptors, CD105 binds to TGF-β1 and TGF-β3 with high affinity, but does not bind TGF-β2. Other ligands reportedly include Activin A, BMP-2, and BMP-7. CD105 has important roles in angiogenesis, cardiovascular development, and vascular remodeling, and the protein serves a regulatory role in cytoskeletal reorganization by modulating the sites of focal adhesion and cellular migration. Certain mutations in CD105 result in the autosomal dominant disorder hereditary hemorrhagic telangiectasia.

This antibody clone has been verified for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411) and MesenCult™-XF Medium (Catalog #05420).
Subtype:
Primary Antibodies
Target Antigen:
CD105 (Endoglin)
Alternative Names:
Endoglin
Reactive Species:
Human; Mouse
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; Unconjugated
Host Species:
Mouse
Cell Type:
Hematopoietic Stem and Progenitor Cells; Mesenchymal Stem and Progenitor Cells
Application:
Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Endothelial Cell Biology; Stem Cell Biology
Clone:
43A3
Gene ID:
2022
Isotype:
IgG1, kappa

Scientific Resources

Educational Materials

(2)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 is shown (solid line histogram).

Data for PE-Conjugated

Figure 2. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, PE is shown (solid line histogram).

Data for Unconjugated

Figure 3. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, followed by a rat anti-mouse IgG1 antibody, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, PE is shown (solid line histogram).

Data for APC-Conjugated

Figure 4. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, APC is shown in the bottom panel (solid line histogram).

Data for Biotin-Conjugated

Figure 5. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC is shown in the bottom panel (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, FITC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, FITC is shown in the bottom panel (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.