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Data and Publications
(A) The biological activity of Mouse Recombinant IL-6 was tested by its ability to promote the proliferation of 7TD1 cells. Cell proliferation
was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 2.4 - 3.6 pg/mL.
(B) 1 μg of Mouse Recombinant IL-6 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-6 has a predicted molecular mass of 21.9 kDa.