Mouse Recombinant GM-CSF (E. coli-expressed)

Granulocyte-macrophage colony-stimulating factor

Mouse Recombinant GM-CSF (E. coli-expressed)

Granulocyte-macrophage colony-stimulating factor

From: 189 USD
Catalog #
78017_C
Granulocyte-macrophage colony-stimulating factor

Overview

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitor cells (Barreda et al.). GM-CSF was first purified from the culture of mouse lung tissue after lipopolysaccharide treatment. GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.).
Subtype
Cytokines
Alternative Names
Colony-stimulating factor 2, CSF-2, MGI-1GM, Pluripoietic-alpha
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Monocytes, Myeloid Cells
Species
Mouse
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
14.3 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Mouse Recombinant GM-CSF was tested by its ability to promote the proliferation of FDC-P1 cells. Cell proliferation was measured after 91 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3 - 5 pg/mL.
(B) 1 μg of Mouse Recombinant GM-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant GM-CSF has a predicted molecular mass of 14.3 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78017, 78017.1, 78017.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78017, 78017.1, 78017.2
Lot #
All
Language
English

Resources and Publications

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