ImmunoCult™ Mouse T Cell Activator Kit

Mouse T cell activation and expansion reagent

ImmunoCult™ Mouse T Cell Activator Kit

Mouse T cell activation and expansion reagent

From: 499 USD
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Mouse T cell activation and expansion reagent
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Product Advantages

  • Activate mouse T cells with confidence using a single kit that allows for flexible design and activation by CD3/CD28 or CD3/CD28/CD2.
  • Achieve optimal activation without the use of magnetic beads, feeder cells, or antigen.
  • Maintain functional T cells with high viability by using a gentle activation stimulus.
  • Rely on a highly stable, filter-sterilized, soluble reagent that is compatible with common T cell growth medium.

What's Included

  • ImmunoCult™ Mouse T Cell Activator Component A, 1.0 mL
  • ImmunoCult™ Mouse T Cell Activator Component B, 1.0 mL
  • ImmunoCult™ Mouse T Cell Activator Component C, 1.0 mL

Overview

Achieve robust activation and expansion of mouse T cells in the absence of magnetic beads, feeder cells, or antigens.

The ImmunoCult™ Mouse T Cell Activator Kit’s gentle activation stimulus encourages high viability of activated T cells, which can be further expanded in common mouse T cell growth media. This kit consists of antibody complexes that bind to and cross-link CD3 and CD28 cell surface ligands, with the option to also target CD2, thereby providing the required primary and co-stimulatory signals for T cell culture and activation.

Optimal T cell activation can be achieved within 2 - 3 days using all 3 kit components (A, B, and C). Component A contains anti-mouse CD3 and CD28 antibodies for the necessary antigen and co-stimulatory activation signals. Component B contains anti-mouse CD2 antibodies for optional targeting. Component C helps to cross-link the antibodies in Component A and B. All reagents are highly stable, filter-sterilized and soluble, removing additional time-consuming plate coating steps during T cell culture, while allowing for a seamless integration into established workflows.

For efficient isolation of mouse T cells, the ImmunoCult™ Mouse T Cell Activator Kit can be combined with the EasySep™ workflow. It is compatible with kits such as EasySep™ Mouse T Cell Isolation Kit (Catalog #19851), EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852), EasySep™ Mouse CD8+ T Cell Isolation Kit (Catalog #19853), EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), EasySep™ Release Mouse CD4+CD304+ Regulatory T Cell Isolation Kit (Catalog #100-1570), and EasySep™ Mouse CD4 Positive Selection Kit II (Catalog #18952; Lung).
Contains
  • Anti-mouse CD3 monospecific antibody
  • Anti-mouse CD28 monospecific antibody 
  • Anti-mouse CD2 monospecific antibody
  • Antibody crosslinking complex
Subtype
Complete Kits
Cell Type
T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Regulatory
Species
Mouse
Application
Activation, Cell Culture, Expansion
Brand
ImmunoCult
Area of Interest
Immunology, Cell Therapy Development

Data Figures

Figure 1. ImmunoCult™ Mouse T Cell Activator Kit Supports High Viability of Activated T Cells

Figure 1. ImmunoCult™ Mouse T Cell Activator Kit Supports High Viability of Activated T Cells

Mouse T cells were isolated using EasySep™ Mouse T Cell Isolation Kit, stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in IMDM + FBS formulation. Following 3 days of culture, the mean ± SD frequency of CD25+ cells was 91.9 ± 5.1% (n = 11) or 99.9 ± 0.1% (n = 5), when stimulated with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, respectively. Stimulated mouse T cells maintained expression levels of CD25 throughout the 7-day culture period.

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

Figure 2. Robust Expansion of EasySep™-Isolated Mouse T Cells Can Be Achieved Following Stimulation with ImmunoCult™ Mouse T Cell Activator Kit

EasySep™-isolated mouse T cells were expanded with ImmunoCult™ Mouse T Cell Activator Kit in IMDM + FBS formulation over 7 days. The number of viable cells was assessed every 2 - 3 days, and fresh medium supplemented with IL-2 was added. No additional ImmunoCult™ Mouse T Cell Activator was added during the 7-day culture period. After 7 days in culture with ImmunoCult™ Mouse CD3/CD28 T Cell Activator or ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator, stimulation resulted in a fold expansion of 23 ± 3.4 or 29.3 ± 4.8 (mean ± SEM, n = 6), respectively.

Figure 3. High Cell Proliferation is Observed in EasySep™-Isolated T cells After Stimulation with  ImmunoCult™ Mouse T Cell Activator

Figure 3. High Cell Proliferation is Observed in EasySep™-Isolated T cells After Stimulation with ImmunoCult™ Mouse T Cell Activator

EasySep™-isolated mouse T cells were labeled with CFDA-SE (Catalog #75003), stimulated with ImmunoCult™ Mouse T Cell Activator Kit, and cultured in cultured in IMDM + FBS formulation. On Day 3, cells were harvested, stained with anti-mouse CD4 and CD8a antibodies, then measured by flow cytometry. Shown are CFDA-SE-labeled mouse T cells, gated on viable CD4+ (A) or CD8a+ (B) cells, cultured with no activator (top panel), with ImmunoCult™ Mouse CD3/CD28 T Cell Activator (middle panel), or with ImmunoCult™ Mouse CD3/CD28/CD2 T Cell Activator (bottom panel). Due to cell proliferation, the intensity of CFDA-SE signal is reduced by 50% for each cell division.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-1572
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-1572
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (2)

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.