CFDA-SE

Cell proliferation and tracking dye

More Views

CFDA-SE

Cell proliferation and tracking dye

10 mg
Catalog #75003
108 USD

Overview

CFDA-SE (5(6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. Upon diffusion into the cell, intracellular esterases cleave the acetate group to generate CFSE, which interacts with cellular amines via its succinimidyl groups to generate a highly fluorescent green dye that is impermeant to the cell membrane. CFDA-SE is frequently used in cell proliferation assays, as it is partitioned approximately equally between the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions. CFDA-SE is also used for motility assays and in vivo cell tracking experiments. CFDA-SE-labeled cells can be detected with any instrument/filter set compatible with fluorescein detection.
Application:
Flow Cytometry; Immunocytochemistry; Immunofluorescence
Area of Interest:
Neuroscience; Immunology; Stem Cell Biology
Chemical Formula:
C₂₉H₁₉NO₁₁
Molecular Weight:
557.5 g/mol

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Educational Materials

(1)

Data and Publications

Data

(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with CFDA-SE and analyzed by flow cytometry after being cultured for 0, 24, and 48 hours (filled histograms). Solid line histogram (control) shows unlabeled cells analyzed after 48 hours of cell culture. (B) Chemical structure of CFDA-SE.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.