Human Recombinant Shh (C24II)
Sonic hedgehog
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Human Recombinant Shh (C24II) -
Label for Human Recombinant Shh (C24II) -
Label for Human Recombinant Shh (C24II) -
Label for Human Recombinant Shh (C24II)
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Overview
Sonic hedgehog (Shh) is a member of the Hedgehog family of secreted signaling proteins that has a prominent role in patterning during early mammalian development (Ho & Scott). It is expressed during early embryogenesis in a variety of tissues (Finco et al.). During patterning of the central nervous system, Shh directly acts on cells to specify neural cell fates, regulates proliferation and survival of oligodendrocytes precursors and neural crest cells and stimulates proliferation, differentiation and axon growth (Ho & Scott; Martí). Shh also regulates T cell differentiation and activation (Crompton et al.). Recombinant human Shh (C24II; rhShh) is a fully biologically active molecule.
Subtype:
Cytokines
Alternative Names:
HHG-1, HLP3, HPE3, MCOPCB5, SMMCI, Sonic hedgehog, TPT, TPTPS
Cell Type:
Pluripotent Stem Cells; Neural Stem and Progenitor Cells; Neural Cells, PSC-Derived
Species:
Human
Area of Interest:
Stem Cell Biology; Neuroscience
Molecular Weight:
19.7 kDa
Purity:
≥ 95%
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Data
(A) The biological activity of Human Recombinant Shh (C24II) was tested by its ability to promote alkaline phosphatase production of
CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example 0.7 μg/mL.
(B) 4 μg of Human Recombinant Shh (C24II) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining. Human Recombinant Shh (C24II) has a predicted molecular mass of 19.7 kDa.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.