Human Recombinant Noggin (E. coli-expressed), ACF

Noggin, animal component-free

Human Recombinant Noggin (E. coli-expressed), ACF

Noggin, animal component-free

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Noggin, animal component-free
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Overview

Use noggin to support the maintenance of undifferentiated human embryonic stem cells in vitro, and to prevent spontaneous differentiation in the short term (Chaturvedi et al. Cell Prolif, 2009). Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin is essential for development of structures derived from ectoderm embryonic somite, skeletal patterning, and neurogenesis in vivo. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al. Int J Biochem Cell Biol, 2011), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al. Stem Cells, 2008).
Subtype
Cytokines
Alternative Names
NOG, SYM1, SYNS1
Cell Type
Neural Cells, PSC-Derived, Neurons, Pluripotent Stem Cells
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98%

Data Figures

Figure 1. Biological Activity and Molecular Mass of Human Recombinant Noggin (E. coli-expressed), ACF

(A) The biological activity of Human Recombinant Noggin (E. coli-expressed), ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells. Inhibition of BMP-2 was measured using a firefly luciferase reporter assay. Cells were treated with a serial dilution of Noggin and standard concentration of BMP-2 for 6 hours. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.5 nM (70.1 ng/mL). (B) 7 μg of Human Recombinant Noggin (E. coli-expressed), ACF was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Noggin has a predicted molecular mass of 23 kDa in reducing conditions.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-1731
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1731
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.