Human Recombinant IL-3, ACF

Interleukin 3, animal component-free
Catalog #
78146_C
Interleukin 3, animal component-free
From: 204 USD

Overview

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
HCGF, Interleukin-3, MCGF, Multi-CSF, P-cell stimulation factor
Cell Type
Hematopoietic Stem and Progenitor Cells, Pluripotent Stem Cells
Area of Interest
Stem Cell Biology
Molecular Weight
15.2 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-3, ACF
Catalog #
78146, 78146.1, 78146.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-3, ACF
Catalog #
78146, 78146.1, 78146.2
Lot #
All
Language
English

Educational Materials(4)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-3, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.244 ng/mL.
(B) 1 μg of Human Recombinant IL-3, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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