Human Recombinant GDNF

Glial cell line-derived neurotrophic factor
Catalog #
78058_C
Glial cell line-derived neurotrophic factor
From: 185 USD

Overview

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knots (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4); GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen & Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increases their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor; Catalog #78005), BrainPhys™ Neuronal Medium (Catalog #05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.).
Subtype
Cytokines
Alternative Names
Astrocyte-derived trophic factor, ATF, ATF1, ATF2, Glial cell-derived neurotrophic factor
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
15.2 kDa monomer; 30.4 kDa dimer
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant GDNF
Catalog #
78058, 78058.1, 78058.2, 78058.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant GDNF
Catalog #
78058, 78058.1, 78058.2, 78058.3
Lot #
All
Language
English

Educational Materials (5)

Brochure
Primary Neuronal Culture: Standardized Media and Reagents
Brochure
BrainPhys™ Neuronal Medium for Improved Neuronal Function
Brochure
hPSC-Derived Neural Cell Research
Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Products for Human Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant GDNF was tested by its ability to promote the proliferation of C6 cells. Cell proliferation was measured after 7 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 2.17 μg/mL. (B) 1 μg of Human Recombinant GDNF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GDNF is a homodimer of 15.2 kDa subunits with a predicted total molecular mass of 30.4 kDa.

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