Human IL-17A ELISA Kit

For detection and measurement of human interleukin 17A
Catalog #
02036_C
For detection and measurement of human interleukin 17A
From: 785 USD

Overview

The Human Interleukin 17A (IL-17A) ELISA Kit is designed for the quantitative detection and measurement of human IL-17A in biological fluids such as serum, plasma, and cell culture supernatants. IL-17A is secreted by Th17 cells, CD8+ T cells, γδ T cells, natural killer T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells. IL-17A is a pro-inflammatory cytokine that plays a key role in the defense against pathogens, activates the NF-kB, MAPK, and C/EBP pathways, and in conjunction with TNF-α, induces sustained neutrophil recruitment during inflammation.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components
  • Human IL-17A ELISA Kit, 2 plates (Catalog #02036)
    • Human IL-17A ELISA Plate, 2x
    • Human IL-17A Standard, 1 vial
    • Human IL-17A Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer C, 1 m
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human IL-17A ELISA Kit, 10 plates (Catalog #02037)
    • Human IL-17A ELISA Plate, 10x
    • Human IL-17A Standard, 1 vial
    • Human IL-17A Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    •  SA-HRP Diluent, 120 mL
    •  TMB Substrate, 120 m
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype
Complete Kits
Cell Type
B Cells, Lymphocytes, Mesenchymal Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+, T Cells, Other Subsets
Species
Human
Area of Interest
Immunology, Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Human IL-17A ELISA Kit
Catalog #
02036, 02037
Lot #
All
Language
English

Educational Materials (2)

Brochure
ELISA Kits for Immunology Research
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable Range: 3.2 - 1000 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 1 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 01/420.
• Recovery: A mid-curve recovery of 96 - 119% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.9% (CV). The inter-assay precision of this assay is 7.3% (CV).

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