EasySep™ Mouse Pan-ILC Enrichment Kit

16-Minute cell isolation using Immunomagnetic negative selection

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Mouse Pan-ILC Enrichment Kit

16-Minute cell isolation using Immunomagnetic negative selection

EasySep™ Mouse Pan-ILC Enrichment Kit
1 x 10^9 cells
801 USD
Catalog # 19875

Immunomagnetic enrichment of untouched mouse ILC1, ILC2, and ILC3s

Product Advantages

• Fast, easy-to-use and column-free
• Isolated cells are untouched
• Facilitates rapid flow sorting of ILCs

What's Included

  • EasySep™ Mouse Pan-ILC Enrichment Kit (Catalog #19875)
    • EasySep™ Mouse Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

This product is designed to enrich group 1, 2 and 3 innate lymphoid cells (ILC1, ILC2 and ILC3s) from single-cell suspensions of mouse tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-ILCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Mouse
Sample Source
Bone Marrow, Lung, Other
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19875
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19875
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19875
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Figure 1. Typical EasySep™ Mouse Pan-ILC Enrichment Profile

Starting with a naïve mouse lung single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 3.1 - 7.6%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.4% and 3.9% (or 0.4% and 6.8% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.6%.

Figure 2. Typical EasySep™ Mouse Pan-ILC Enrichment Profile from Mouse Lymph Node

Starting with a naïve mouse lymph node single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 21.1 - 45.2%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.3% and 17.6% (or 0.3% and 24.7% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.4%.

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