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Anti-Mouse TER119 Antibody, Clone TER-119

Rat (WI) monoclonal IgG2b antibody against mouse TER119

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Anti-Mouse TER119 Antibody, Clone TER-119

Rat (WI) monoclonal IgG2b antibody against mouse TER119

From: 77 USD
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Rat (WI) monoclonal IgG2b antibody against mouse TER119
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Overview

The TER-119 antibody reacts with murine TER119 (Ly-76), an ~52 kDa protein associated with glycophorin A on the surface of cells of the erythroid lineage in embryonic yolk sac, fetal and newborn liver, adult bone marrow, peripheral blood, and lymphoid organs. TER119 is an erythroid-specific marker expressed at all stages of differentation from early proerythroblasts to mature erythrocytes, but not by erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid). The TER-119 antibody is a component of the "lineage cocktail" used to detect, or deplete cells committed to hematopoietic lineages. In adult mice, TER119 is found on ~20 - 25% of bone marrow cells and ~2 - 3% of splenocytes.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse CD4+ T Cell Isolation Kit (Catalog #19852).
Subtype
Primary Antibodies
Target Antigen
TER119
Alternative Names
Ly-76, TER-119
Reactive Species
Mouse
Conjugation
Biotin, FITC, PE, PerCP-Cyanine5.5
Host Species
Rat
Cell Type
Erythroid Cells, Red Blood Cells
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
TER-119
Gene ID
104231
Isotype
IgG2b, kappa

Data Figures

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, followed by goat antimouse IgG, FITC.
(B) Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with a rat IgG2b, kappa isotype control antibody followed by goat anti-mouse IgG, FITC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488 (filled histogram) or a rat IgG2b, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Image showing flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE.

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER-119 Antibody, Clone TER-119, Biotin followed by streptavidin (SAV) PE (filled histogram) or a rat IgG2b, kappa isotype control antibody, Biotin followed by streptavidin (SAV) PE (solid line histogram).

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 5. Data for APC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, APC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (Catalog #60077AZ) (solid line histogram).

Data for FITC-Conjugated

Figure 6. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, FITC (filled histogram) or Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (Catalog #60077FI) (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells labeled with Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5 (filled histogram) or a rat IgG2b, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC
Catalog #
60033FI, 100-0453, 100-0454
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin
Catalog #
100-0456, 60033BT.3, 100-0455
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488
Catalog #
60033AD
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5
Catalog #
100-0458, 100-0457
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC
Catalog #
60033AZ.1, 60033AZ
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE
Catalog #
100-0452, 100-0451
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119
Catalog #
60033
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, FITC
Catalog #
60033FI, 100-0453, 100-0454
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Biotin
Catalog #
100-0456, 60033BT.3, 100-0455
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, Alexa Fluor® 488
Catalog #
60033AD
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PerCP-Cy5.5
Catalog #
100-0458, 100-0457
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, APC
Catalog #
60033AZ.1, 60033AZ
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119, PE
Catalog #
100-0452, 100-0451
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Mouse TER119 Antibody, Clone TER-119
Catalog #
60033
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (4)

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Reliable Antibodies for Your Research
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Tools for Your Immunology Research
Hematopoietic Stem and Progenitor Cells - Products for Your Research
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Hematopoietic Stem and Progenitor Cells - Products for Your Research
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Wallchart
Human Immune Cytokines

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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