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Due to pandemic-related plasticware shortages, you may see temporary changes to product packaging. Product quantity and quality are unaffected.

EasySep™ Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection cell enrichment kit

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human Pan-ILC Enrichment Kit

Immunomagnetic negative selection cell enrichment kit

From: 943 USD
Catalog #
17975_C
Immunomagnetic negative selection cell enrichment kit

Product Advantages


  • Fast, easy-to-use and column-free

  • Untouched, viable cells

  • Facilitates rapid flow sorting of ILCs

What's Included

  • EasySep™ Human Pan-ILC Enrichment Kit (Catalog #17975)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
  • RoboSep™ Human Pan-ILC Enrichment Kit (Catalog #17975RF)
    • EasySep™ Human Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50103, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Leukapheresis, PBMC
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Typical EasySep™ Human Pan-ILC Enrichment Profile

Figure 1. Typical EasySep™ Human Pan-ILC Enrichment Profile

Starting with fresh leukapheresis samples, the total ILC content (Lin-CD45+CD127+) of the enriched fraction typically ranges from 17 - 85%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.09% and 45% (or 0.1% and 54% of CD45+ cells), respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human Pan-ILC Enrichment Kit
Catalog #
17975
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human Pan-ILC Enrichment Kit
Catalog #
17975RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (13)

Tools For Your Immunology Research
Brochure
Tools For Your Immunology Research
Cell Enrichment Prior to Cell Sorting
Brochure
Cell Enrichment Prior to Cell Sorting
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Gating Strategy for the Identification of ILC2s
Technical Bulletin
Gating Strategy for the Identification of ILC2s
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Innate Lymphoid Cells
Wallchart
Innate Lymphoid Cells
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
0:57
Video
Simultaneous Cell Isolation from Multiple Samples Using the EasyEights™ EasySep™ Magnet
How to Pipet Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Magnet
1:16
Video
How to Pipet Off Samples During Cell Isolation Using the EasyEights™ EasySep™ Cell Separation Ma...
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
1:23
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality
Scientific Poster
Pre-enrichment of Human ILCs Improves Sorting Efficiency While Maintaining Functionality
Efficient Enrichment of Functional ILC Subsets from Human PBMCs by Immunomagnetic Selection
Scientific Poster
Efficient Enrichment of Functional ILC Subsets from Human PBMCs by Immunomagnetic Selection

Related Products

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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