EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

18-Minute cell isolation kit using immunomagnetic negative selection

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EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

Immunomagnetic isolation of untouched myeloid-derived suppressor cells (CD11b+Gr1+).

1 x 109 cells
Catalog #19867
667 USD

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Required Products

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit is designed to isolate CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-MDSCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell sorting, and cell culture.
Advantages:
• Fast, easy-to-use and column-free
• Up to 96% purity
• Untouched, viable cells
Components:
  • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit (Catalog #19867)
    • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.2 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets; Monocytes; Myeloid Cells
Species:
Mouse
Sample Source:
Bone Marrow; Other; Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(7)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
2:12
How to Prepare a Single-Cell Suspension from Mouse Spleen
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
0:57
EasyEights™: Faster and Easier Multiple Sample Cell Isolations

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
Granulocyte Research
Cell Sourcing
Cell Isolation
Cell Characterization
Monocyte Research
Cell Sourcing
Cell Isolation
Differentiation and Maturation
Cell Characterization
Myeloid Cell Research
Cell Sourcing
Cell Isolation
Cell Characterization

Data and Publications

Data

Figure 1. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from 4T1 Tumor-Bearing BALB/c Mouse Splenocytes

Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 20.7% and 94.7%, respectively.

Figure 2. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from Naïve C57BL/6 Mouse Splenocytes

Starting with naïve C57BL/6 mouse splenocytes, the CD11b+Gr1+ cell content of the isolated fraction is typically 86 ± 4.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.0% and 86.8%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.