EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Immunomagnetic cell isolation kit using particle release technology
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This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
Advantages
• Easy-to-use and column-free
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components
EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)
EasySep™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
EasySep™ Dextran RapidSpheres™, 1 mL
EasySep™ Human CD62L Positive Selection Cocktail, 1 mL
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Video
1:57
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Scientific Poster
Immunomagnetic Purification of Human Central and Effector Memory T Cell Subsets in 45 Minutes
Product Applications
This product is designed for use in the following research area(s) as part
of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we
offer to support each research area.
Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.
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EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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