EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
Immunomagnetic cell isolation kit using particle release technology
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EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit -
Label for EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
Advantages:
• Easy-to-use and column-free
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
- EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)
- EasySep™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
- EasySep™ Dextran RapidSpheres™, 1 mL
- EasySep™ Human CD62L Positive Selection Cocktail, 1 mL
- EasySep Human CD25 Depletion Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasyeSep™ Release Buffer, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+
Species:
Human
Sample Source:
PBMC; Leukapheresis
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
Related Products
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Human Peripheral Blood Leukopak, Fresh
Primary human cells, fresh -
EasySep™ Human Central and Effector Memory CD8+ T Cell Isolation Kit
Immunomagnetic cell isolation kit using particle release technology
Labeling Antibodies
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Anti-Human CD3 Antibody, Clone SK7
Mouse monoclonal IgG1 antibody against human, chimpanzee CD3 -
Anti-Human CD45RO Antibody, Clone UCHL1
Mouse monoclonal IgG2a antibody against human, chimpanzee, common marmoset CD45RO -
Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
Scientific Resources
Product Documentation
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17865
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17865
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17865
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Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.