EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit

Immunomagnetic cell isolation kit using particle release technology

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EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit

Immunomagnetic positive selection of human central and effector memory CD4+ T cells

1 x 109 cells
Catalog #17865
806 USD

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Required Products

Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

This kit isolates human CD62L+CD45RO+ and CD62L-CD45RO+ memory CD4+ T cells from fresh peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples. First, CD62L+ cells are isolated by column-free immunomagnetic positive selection using antibody complexes and EasySep™ Releasable RapidSpheres™. Then, bound magnetic particles are removed from the EasySep™-isolated CD62L+ cells, and unwanted CD45RA+ and non-CD4+ T cells are targeted for depletion using antibody complexes and EasySep™ Dextran RapidSpheres™. The final isolated fraction contains highly purified CD62L+CD45RO+ central memory CD4+ T cells that are immediately ready for downstream applications. Effector memory CD4+ T cells can be isolated from the CD62L- fraction.
Advantages:
• Easy-to-use and column-free
• Up to 96% purity
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
  • EasySep™ Human Central and Effector Memory CD4+ T Cell Isolation Kit (Catalog #17865)
    • EasySep™ Human Memory CD4+ T Cell Isolation Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™, 1 mL
    • EasySep™ Human CD62L Positive Selection Cocktail, 1 mL
    • EasySep Human CD25 Depletion Cocktail, 1 mL
    • EasySep™ Releasable RapidSpheres™, 1 mL
    • EasyeSep™ Release Buffer, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD4+
Species:
Human
Sample Source:
PBMC; Leukapheresis
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology

Scientific Resources

Product Documentation

Educational Materials

(9)
Brochure
Tools For Your Immunology Research
Brochure
EasySep™ Cell Separation Technology
Wallchart
Antigen Processing and Presentation
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Human Immune Cytokines
Video
1:57
Fast and Easy Cell Isolation with EasySep™
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Scientific Poster
Immunomagnetic Purification of Human Central and Effector Memory T Cell Subsets in 45 Minutes

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
T Cell Engineering
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Cell Cryopreservation

Data and Publications

Data


Starting with fresh PBMCs, the central memory CD4+ T cell content (CD3+CD4+CD45RO+CD62L+) of the isolated fraction is typically 92.3 ± 3.9% (mean ± SD using the purple EasySep™ magnet). The effector memory content (CD3+CD4+CD45RO+CD62L-) of the isolated fraction is typically 92.4 ± 4.1% (mean ± SD).
NOTE: RBCs were removed from the start sample by lysis prior to flow cytometry.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.