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EasySep™ Human CD33 Positive Selection Kit II

Immunomagnetic positive selection of human CD33+ cells (e.g. myeloid cells)

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ Human CD33 Positive Selection Kit II

Immunomagnetic positive selection of human CD33+ cells (e.g. myeloid cells)

From: 832 USD
Catalog #
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Immunomagnetic positive selection of human CD33+ cells (e.g. myeloid cells)
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Product Advantages


  • Fast and easy-to-use

  • Up to 98% purity

  • No columns required

  • Compatible across EasySep™, "The Big Easy", and RoboSep™-S platforms

What's Included

  • EasySep™ Human CD33 Positive Selection Kit II (Catalog #17876)
    • EasySep™ Human CD33 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
  • RoboSep™ Human CD33 Positive Selection Kit II with Filter Tips (Catalog #17876RF)
    • EasySep™ Human CD33 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily isolate highly purified human CD33+ cells from ammonium chloride-lysed blood or bone marrow, or from nucleated cells prepared by density separation with RosetteSep™ DM-M density medium (Catalog #15725) samples. Use immunomagnetic positive selection with the EasySep™ Human CD33 Positive Selection Kit II. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD33 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD33+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.

This product replaces the EasySep™ Human CD33 Positive Selection Kit (Catalog #18257) for even faster cell isolations.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™. Explore additional products optimized for your workflow, including culture media, supplements, antibodies, and more.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Human
Sample Source
PMNC
Selection Method
Positive
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with ammonium chloride-lysed peripheral blood, the CD33+ cell content of the isolated fraction is typically 95.6 ± 1.6%, as assessed by labeling with CD14 and CD66b (mean + SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 44.7% and 96.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Human CD33 Positive Selection Kit II
Catalog #
17876RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human CD33 Positive Selection Kit II
Catalog #
17876
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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