EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
Immunomagnetic positive selection of human CD15+ cells from fresh human whole blood or buffy coat samples

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In-lab demonstration
- "The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation
- EasySep™ Buffer
Cell separation buffer for use with EasySep™ cell separation kits
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Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells
Overview
In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD15 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD15+ cells are ready for downstream applications such as flow cytometry, culture, or DNA/RNA extraction for lineage-specific chimerism analysis. The CD15 antigen is expressed on granulocytes and monocytes.
Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Data Figures

Starting with human whole blood, the CD15+ cell content of the isolated fraction is typically 99.2 ± 1.1% (gated on CD45; mean ± SD using the “Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 57.6% and 99.9%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (10)
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- RoboSep™-S
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Labeling Antibodies
- Anti-Human CD14 Antibody, Clone M5E2
Mouse monoclonal IgG2a antibody against human, rhesus, cynomolgus CD14
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EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.