EasySep Human CD2 Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit

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From: 667 USD

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Immunomagnetic positive selection cell isolation kit
From: 667 USD

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Overview Related Products Scientific Resources Product Applications Data and Publications

Overview

The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.

This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 97% purity
• No columns required
Components:
  • EasySep™ Human CD2 Positive Selection Kit (Catalog #17883)
    • EasySep™ Human CD2 Positive Selection Cocktail II, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
  • RoboSep™ Human CD2 Positive Selection Kit (Catalog #17883RF)
    • EasySep™ Human CD2 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50101, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology

Scientific Resources

Product Documentation

Educational Materials

(10)
Brochure
Isolate Human Immune Cells
Brochure
EasySep™ Cell Separation Technology
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
The Immune Response to HIV Poster
Video
1:13
The EasySep™ Pour-off: A Simple and Effective Way to Isolate Cells
Video
0:57
EasyEights™: Faster and Easier Multiple Sample Cell Isolations
Video
1:37
How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
Video
1:57
Fast and Easy Cell Isolation with EasySep™

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages
T Cell Research
Cell Sourcing
Cell Isolation
Cell Activation and Expansion
Cell Characterization
T Cell Engineering
Cell Isolation
Cell Activation and Expansion
Cell Characterization
Cell Cryopreservation

Data and Publications

Data

Starting with PBMCs, the CD2+ cell content of the isolated fraction is typically 93.8 ± 3.3% (mean ± SD using the purple EasySep™ Magnet; as assessed by labeling with CD3 and CD56). In the above example, the purities of the start and final isolated fractions are 66.1% and 94.8%, respectively.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.