EasySep Human CD2 Positive Selection Kit II
Immunomagnetic positive selection cell isolation kit
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EasySep Human CD2 Positive Selection Kit II -
EasySep Human CD2 Positive Selection Kit II -
Label for EasySep Human CD2 Positive Selection Kit II
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Human CD2 Positive Selection Kit II is designed to isolate CD2+ cells from fresh or previously frozen human peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD2 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD2 antigen is expressed on T cells and NK cells.
This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
This product replaces the EasySep™ Human CD2 Positive Selection Kit (Catalog #18657) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 97% purity
• No columns required
• Up to 97% purity
• No columns required
Components:
- EasySep™ Human CD2 Positive Selection Kit (Catalog #17883)
- EasySep™ Human CD2 Positive Selection Cocktail II, 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 1 mL
- RoboSep™ Human CD2 Positive Selection Kit (Catalog #17883RF)
- EasySep™ Human CD2 Positive Selection Cocktail, 1 mL
- EasySep™ Dextran RapidSpheres™ 50101, 1 mL
- RoboSep™ Buffer (Catalog #20104)
- RoboSep™ Filter Tips (Catalog #20125)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology
Related Products
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EasySep™ Human CD4 Positive Selection Kit II
Immunomagnetic positive selection kit -
Density gradient medium for the isolation of mononuclear cells -
EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit
Immunomagnetic positive selection cell isolation kit -
EasySep™ Human CD3 Positive Selection Kit II
Immunomagnetic positive selection kit
Scientific Resources
Product Documentation
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data and Publications
Data
Starting with PBMCs, the CD2+ cell content of the isolated fraction is typically 93.8 ± 3.3% (mean ± SD using the purple EasySep™ Magnet; as assessed by labeling with CD3 and CD56). In the above example, the purities of the start and final isolated fractions are 66.1% and 94.8%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.