EasySep™ Human CD11b Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

EasySep™ Human CD11b Positive Selection and Depletion Kit

Immunomagnetic positive selection and cell depletion kit

From: 695 USD
Catalog #
100-0742_C
Immunomagnetic positive selection and cell depletion kit

Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0742)
    • EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • RoboSep™ Human CD11b Positive Selection and Depletion Kit (Catalog #100-0744)
    • EasySep™ Human CD11b Positive Selection Depletion Cocktail, 0.75 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Tips (Catalog #20125)

Overview

Efficiently isolate or deplete human CD11b+ cells from fresh or previously frozen peripheral blood mononuclear cells (PBMCs) or leukapheresis samples in as little as 16 minutes. Cells isolated with the EasySep™ Human CD11b Positive Selection and Depletion Kit can be used immediately in downstream applications such as flow cytometry, cell culture, or DNA/RNA extraction.

Use this kit to label CD11b+ cells with antibodies and magnetic particles. The cells are then subsequently separated—without the use of columns—using an EasySep™ magnet. Labeled cells remain in the tube while unlabeled cells are poured off. Depending on your research needs, either fraction can be retained for subsequent applications.

This kit targets human CD11b, a subunit of the integrin macrophage-1 antigen (Mac-1) receptor commonly used as a myeloid cell marker. CD11b is expressed on immune cells such as monocytes, macrophages, neutrophils, and NK cell subsets, and is known to play a role in regulating cell adhesion, migration, and phagocytosis (Khan SQ et al. Front Med, 2018).

Learn more about how immunomagnetic EasySep™ technology works.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Macrophages, Monocytes, NK Cells
Species
Human
Sample Source
Leukapheresis, Peripheral Blood
Selection Method
Depletion, Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology, Infectious Diseases

Data Figures

Typical EasySep™ Human CD11b+ Positive Selection Profile

Figure 1. Typical EasySep™ Human CD11b+ Positive Selection Profile

Starting with human PBMCs, the CD11b+ cell content of the isolated fraction is typically 98.6 ± 1.4% after positive selection and 1.7 ± 1.2% after depletion (mean ± SD using the EasySep™ magnet). In the above example, the frequencies of CD11b+ cells in the start, final isolated, and final depleted fractions are 47.8%, 99.4%, and 1.6%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0742
Lot #
All
Language
English
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0742
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0744
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
100-0744
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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