Overview
The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.
This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
Advantages:
• Fast and easy-to-use
• Up to 99.9% purity
• No columns required
• Up to 99.9% purity
• No columns required
Components:
- EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit (Catalog #17889)
- EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit with Filter Tips (Catalog #17889RF)
- EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL
- EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL
- EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
- RoboSep™ Buffer (Catalog #20104) x 2
- RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells, CD8+
Species:
Human
Sample Source:
Buffy Coat; Whole Blood
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep; RoboSep
Area of Interest:
Chimerism; HLA; Immunology
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
17889
All
17889
All
17889RF
All
17889RF
All
17889RF
All
17889RF
All
17889RF
All
Educational Materials
(12)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data

Starting with human whole blood, the total CD8+ cell content of the isolated fraction is typically 98.7 ± 1.1% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 2.4% and 95.8%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.