EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit

From: 734 USD
Catalog #
17889_C
Immunomagnetic positive selection cell isolation kit

Product Advantages


  • Fast and easy-to-use

  • Up to 99.9% purity

  • No columns required

What's Included

  • EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit (Catalog #17889)
    • EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit with Filter Tips (Catalog #17889RF)
    • EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50101), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

The EasySep™ HLA Chimerism Whole Blood CD8 Positive Selection Kit is designed to isolate CD8+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD8 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD8 antigen is expressed on cytotoxic T cells and weakly on a subset of NK cells.

This product replaces the EasySep™ Human Whole Blood CD8 Positive Selection Kit (Catalog #18083) for even faster cell isolations.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD8+
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with human whole blood, the total CD8+ cell content of the isolated fraction is typically 98.7 ± 1.1% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 2.4% and 95.8%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
17889
Lot #
1000064863 or lower
Language
English
Catalog #
17889
Lot #
1000064864 or higher
Language
English
Catalog #
17889
Lot #
1000064864 or higher
Language
English
Catalog #
17889RF
Lot #
1000064863 or lower
Language
English
Catalog #
17889RF
Lot #
1000064863 or lower
Language
English
Catalog #
17889RF
Lot #
1000064864 or higher
Language
English
Catalog #
17889RF
Lot #
1000064864 or higher
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17889
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17889
Lot #
All
Language
English
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Safety Data Sheet 3
Catalog #
17889
Lot #
All
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English
Document Type
Safety Data Sheet 4
Catalog #
17889
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
17889RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
17889RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
17889RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
17889RF
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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