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EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit

Immunomagnetic positive selection of human CD4+ cells from fresh human whole blood, buffy coat, or an LRS cone

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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit

Immunomagnetic positive selection of human CD4+ cells from fresh human whole blood, buffy coat, or an LRS cone

From: 778 USD
Catalog #
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Immunomagnetic positive selection of human CD4+ cells from fresh human whole blood, buffy coat, or an LRS cone
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Product Advantages


  • Fast and easy-to-use

  • Up to 99% purity

  • No columns required

What's Included

  • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit (Catalog #17888)
    • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
  • RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit with Filter Tips (Catalog #17888RF)
    • EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Cocktail, 3 x 1 mL
    • EasySep™ Dextran RapidSpheres™ (Catalog #50100), 3 x 1 mL
    • EasySep™ RBC Lysis Buffer 10X Concentrate, 10 mL (Catalog #20110)
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. The Big Easy EasySep™ Magnet
    "The Big Easy" EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. EasySep™ Buffer
    EasySep™ Buffer

    Cell separation buffer for use with EasySep™ cell separation kits

Overview

Easily isolate highly purified human CD4+ cells from fresh human whole blood, buffy coat, or a leukoreduction system chamber (LRSC; also known as an LRS cone), using immunomagnetic positive selection, with the EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit. Widely used in published research for more than 20 years, EasySep™ combines the specificity of monoclonal antibodies with the simplicity of a column-free magnetic system.

In this EasySep™ positive selection procedure, desired cells are labeled with antibody complexes recognizing CD4 and magnetic particles. Labeled cells are separated using an EasySep™ magnet and by simply pouring or pipetting off the unwanted cells. The cells of interest remain in the tube. Following magnetic cell isolation, the desired CD4+ cells are ready for downstream applications such as flow cytometry, culture, DNA/RNA extraction, or lineage-specific chimerism analysis. The CD4 antigen is expressed on helper T cells and weakly on monocytes and tissue macrophages.

Learn more about how immunomagnetic EasySep™ technology works or how to fully automate immunomagnetic cell isolation with RoboSep™ to save time and increase laboratory throughput. Explore additional products optimized for your workflow, including those for cell characterization, cryopreservation, and more.
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep Magnet™ (Catalog #18103)
• RoboSep™-S (Catalog #21000)
 
Subtype
Cell Isolation Kits
Cell Type
T Cells, CD4+
Species
Human
Sample Source
Buffy Coat, Peripheral Blood
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Chimerism, HLA, Immunology

Data Figures

Starting with human whole blood, the CD4+CD14- cell content of the isolated fraction is typically 97.0 ± 1.8% (gated on CD45; mean ± SD using “The Big Easy” EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 29.8% and 99.0%, respectively. NOTE: RBCs were removed from the start sample by ammonium chloride lysis prior to flow cytometry.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Product Information Sheet - Buffy Coat or Whole Blood
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English
Document Type
Product Information Sheet - Leukoreduction System Chamber/Cone
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
RoboSep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ HLA Chimerism Whole Blood CD4 Positive Selection Kit
Catalog #
17888
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (12)

Cell Separation Products for HLA Analysis
Brochure
Cell Separation Products for HLA Analysis
EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Technical Bulletin
Fully Automated, High-Purity Cell Isolation for Chimerism Labs
Frequencies of Human Cell Types in Blood-Related Sources
Wallchart
Frequencies of Human Cell Types in Blood-Related Sources
Human Immune Cytokines
Wallchart
Human Immune Cytokines
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
4:55
Video
How to Recover Cells from a Leukocyte Reduction System (LRS) Cone
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
How to Prepare a Buffy Coat from Whole Blood
3:46
Video
How to Prepare a Buffy Coat from Whole Blood
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
56:02
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subsets in as Little as 15 Minutes for Use in Lineage-Specific Chimerism Analysis
Scientific Poster
Isolation of Very Pure Lymphoid (CD3+, CD19+ or CD56+) and Myeloid (CD15+ or CD33/CD66b+) Cell Subse...

Related Products

Related Products
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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