EasySep™ Direct Human T Cell Isolation Kit
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EasySep™ Direct Human T Cell Isolation Kit -
Label for EasySep™ Direct Human T Cell Isolation Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Dulbecco’s phosphate-buffered saline without calcium and magnesium -
Compatible antibodies for purity assessment of isolated cells
Overview
This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
• Up to 97% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
- EasySep™ Direct Human T Cell Isolation Kit (Catalog #19661)
- EasySep™ Direct Human T Cell Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
Related Products
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EasySep™ Human T Cell Isolation Kit
8-Minute cell isolation kit using immunomagnetic negative selection -
Primary human cells, fresh
Labeling Antibodies
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Anti-Human CD4 Antibody, Clone OKT4
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4 -
Anti-Human CD8a Antibody, Clone RPA-T8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a -
Anti-Human CD3 Antibody, Clone UCHT1
Mouse monoclonal IgG1 antibody against human, chimpanzee CD3
Scientific Resources
Product Documentation
Educational Materials
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Frequently Asked Questions
Can EasySep™ be used for either positive or negative selection?
How does the separation work?
Which columns do I use?
How can I analyze the purity of my enriched sample?
Can EasySep™ separations be automated?
Can EasySep™ be used to isolate rare cells?
Are the EasySep™ magnetic particles FACS-compatible?
Can the EasySep™ magnetic particles be removed after enrichment?
Can I alter the separation time in the magnet?
For positive selection, can I perform more than 3 separations to increase purity?
How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?
If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Typical EasySep™ Direct Human T Cell Isolation Profile
Starting with human whole blood from normal healthy donors, the typical T cell (CD3+) content of the non-lysed final isolated fraction is 95.3 ± 1.4% (gated on CD45) or 94.9 ± 1.5% (not gated on CD45). In the example above, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 33.0% and 95.0% (gated on CD45), respectively, or 33.0% and 94.9% (not gated on CD45), respectively. The starting frequency of T cells in the non-lysed whole blood start sample above is 0.059% (data not shown).