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This monoclonal antibody (clone A20) reacts with mouse alloantigen cluster of differentiation 45.1 (CD45.1), also known as Ly5.1 and leukocyte common antigen, a 180 - 240 kDa type I transmembrane glycoprotein. CD45.1 is an allelic form of CD45 expressed by all hematopoietic cells except mature erythrocytes and platelets in mouse strains DA, RIII, SJL/J, and STS/A. Use this recombinant monoclonal antibody in applications requiring detection of CD45.1, such as flow cytometry (FC). This antibody clone has been verified for purity assessment of cells isolated with EasySep™ Mouse CD45 Positive Selection Kit (Catalog #18945) or EasySep™ Mouse TIL (CD45) Positive Selection Kit (Catalog #100-0350).
Clone A20 does not react with leukocytes that express CD45.2 alloantigen. CD45 is a member of the protein tyrosine phosphatase (PTP) family and contains two catalytic domains in the intracellular (COOH) domain, which regulate cellular signaling. The extracellular domains are highly variable due to alternative splicing among exons 4, 5, and 6 as well as differential glycosylation. These CD45 isoforms correlate to specific cell types as well as specific activation and maturation states of immune cells. This monoclonal antibody has been used extensively in adoptive cell transfer and bone marrow transplantation studies in mice, allowing for the differentiation of CD45.2+ and CD45.1+ cells.
Subtype
Primary Antibodies
Target Antigen
CD45.1
Reactive Species
Mouse
Conjugation
APC, Biotin, FITC, PE
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Myeloid Cells
Flow cytometry analysis of SLJ mouse splenocytes labeled with Anti-Mouse CD45.1 Antibody, Clone A20, Biotin followed by streptavidin (SAV) PE (filled histogram).
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